Biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin by a newly isolated strain from a karst area of Guilin, China
文献类型: 外文期刊
第一作者: Wang, Yanchao
作者: Wang, Yanchao;Li, Xia;Sun, Hao;Hao, Zaibin;Wang, Yanchao;Hao, Zaibin;Yi, Kexian;Zheng, Jinlong;Zhang, Jie
作者机构:
关键词: sisal;steroidal saponins;biotransformation;acid hydrolysis;tigogenin
期刊名称:BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT ( 影响因子:1.632; 五年影响因子:2.029 )
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收录情况: SCI
摘要: A rod-shaped bacterium was isolated from the soil in a karst area of Guilin, China and its biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin was presented for the first time. A total of 22 strains for the degradation of steroidal saponins in sisal were isolated from 48 soil samples, and the isolated rod-shaped, bacterial strain ZG-21 was used for the production of tigogenin due to its highest degradation efficiency of steroidal saponins in sisal. The parameters affecting biotransformation by strain ZG-21 were optimized. Under the optimized conditions of temperature (30 degrees C), pH (6), time (5days) and substrate concentration (5mg/mL), a maximum tigogenin yield of 26.7mg/g was achieved. Compared with the conventional method of acid hydrolysis, the biotransformation method provided a clean and eco-friendly alternative for the production of tigogenin.
分类号: Q
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