Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita

文献类型: 外文期刊

第一作者: Yao, Yu-Rong

作者: Yao, Yu-Rong;Tian, Xue-Liang;Shen, Bao-Ming;Mao, Zhen-Chuan;Chen, Guo-hua;Xie, Bing-Yan;Tian, Xue-Liang;Shen, Bao-Ming

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关键词: Acremonium implicatum;Biocontrol;Endophytic;Fungus;gfp;Parasitic;Root-knot nematode

期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )

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收录情况: SCI

摘要: Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum.

分类号: Q93

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