Novel L-lactic acid biosensors based on conducting polypyrrole-block copolymer nanoparticles

文献类型： 外文期刊

第一作者： Sun, Chong

作者： Sun, Chong;Wang, Daoying;Zhang, Muhan;Geng, Zhiming;Xu, Weimin;Liu, Fang;Ni, Yanxiu;Shen, Xiaohui;Song, Youchao;Mao, Chun

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期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）

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收录情况： SCI

摘要： The development of advanced nanomaterials for the highly efficient electrical detection of biological species has attracted great attention. Here, novel polypyrrole-Pluronic F127 nanoparticles (PPy-F127 NPs) with conducting and biocompatibility properties were synthesized and used to construct a L-lactic acid biosensor that could be applied in biochemical assays. The PPy-F127 NPs were characterized by transmission electron microscopy (TEM), elemental analysis and UV-vis spectroscopy. Lactate oxidase (LOx) structure variation on the PPy-F127 NPs was investigated by circular dichroism (CD). The cyclic voltammetric results indicated that LOx immobilized on the PPy-F127 NPs exhibited direct electron transfer reaction with a formal potential value (E-0') of 0.154 V vs. SCE. Moreover, the biosensor had good electrocatalytic activity toward L-lactic acid with a wide linear range (0.015-37.5 mM) and a low detection limit of 0.0088 mM. The regression equation was I (mu A) = 0.02353c (mM) + 1.4135 (R-2 = 0.9939). The L-lactic acid biosensor had a good anti-interference property towards uric acid (UA), ascorbic acid (AA), glucose and cysteine. The idea and method provide a promising platform for the rapid development of biosensors that can be used in the detection of biological species.

分类号： O65

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