Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing

文献类型： 外文期刊

第一作者： Zhang, Ling

作者： Zhang, Ling;Yang, Xiang-dong;Zhang, Yuan-yu;Yang, Jing;Qi, Guang-xun;Guo, Dong-quan;Xing, Guo-jie;Yao, Yao;Xu, Wen-jing;Li, Hai-yun;Li, Qi-yun;Dong, Ying-shan

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期刊名称：INTERNATIONAL JOURNAL OF GENOMICS （ 影响因子：2.326； 五年影响因子：2.557 ）

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收录情况： SCI

摘要： The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybean GmFad2-1b 801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoter BCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression of GmFad2-1b in the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to <3%) in their seed oil content. No structural differences were observed between the fatty acids of the transgenic and the nontransgenic oil extracts.

分类号： Q7

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