Inhibition of isoflavone biosynthesis enhanced T-DNA delivery in soybean by improving plant-Agrobacterium tumefaciens interaction

文献类型: 外文期刊

第一作者: Zhang, Yan-Min

作者: Zhang, Yan-Min;Zhang, Hong-Mei;Liu, Zi-Hui;Guo, Xiu-Lin;Li, Hui-Cong;Li, Guo-Liang;Jiang, Chun-Zhi;Zhang, Meng-Chen

作者机构:

关键词: Soybean;Isoflavones;Inhibition;Transformation;Agrobacterium tumefaciens

期刊名称:PLANT CELL TISSUE AND ORGAN CULTURE ( 影响因子:2.711; 五年影响因子:2.73 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Soybean is a difficult crop to manipulate through Agrobacterium tumefaciens-mediated genetic transformation. Plant-bacterium interaction plays an important role in the transformation process. Being rich with isoflavones, soybean may have an adverse effect on the A. tumefaciens-mediated genetic transformation. To investigate whether high content of endogenous isoflavones in soybean is a serious obstacle in achieving high efficient Agrobacterium-mediated transformation in soybean, a series of experiments on inhibition of isoflavone biosynthesis were conducted to improve upon soybean transformation efficiency. Results indicated that soybean isoflavones inhibited A. tumefaciens growth and respiration, the transformation efficiency [beta-glucuronidase (GUS) transient expression] was negatively correlated with the phenylalanine ammonia-lyase activity and isoflavones content. The biosynthesis of soybean isoflavones was partially inhibited by sonication treatment and applying antagonists in co-culture medium and thereby decreased the adverse effects of isoflavones on Agrobacterium infection. A discernible improvement in transformation efficiency was achieved when sonication at 40 kHz for 3 min was applied along with Agro-infection and the explants were cultured on co-culture medium containing 20 mu M alpha-aminooxyacetic acid (AOA), with the percentage of GUS transient expression as 41.4 %, being 3.6 times higher than that not sonicated and co-cultured on medium without AOA. Sonication was found not only to simply make micro-wounds for Agrobacterium to penetrate or releasing phenolic compounds for induced Agrobacterium vir gene expression; it disturbed the biosynthesis of isoflavones at the transcription level and decreased the adverse effects of isoflavones on soybean transformation, and thereby improving soybean transformation efficiency.

分类号: Q942

  • 相关文献

[1]Overexpression of TaNHX2 enhances salt tolerance of 'composite' and whole transgenic soybean plants. Cao, Dong,Liu, Xiaobing,Cao, Dong,Hou, Wensheng,Liu, Wei,Yao, Weiwei,Wu, Cunxiang,Han, Tianfu,Cao, Dong. 2011

[2]Improvement of soybean transformation via Agrobacterium tumefaciens methods involving alpha-aminooxyacetic acid and sonication treatments enlightened by gene expression profile analysis. Zhang, Yan-Min,Liu, Zi-Hui,Yang, Rui-Juan,Li, Guo-Liang,Guo, Xiu-Lin,Zhang, Hua-Ning,Zhang, Hong-Mei,Di, Rui,Zhao, Qing-Song,Zhang, Meng-Chen.

[3]Improvement of Agrobacterium-mediated transformation efficiency of maize (Zea mays L.) genotype Hi-II by Optimizing Infection and Regeneration Conditions. Xu, You,Ren, Wen,Liu, Ya,Zhao, Jiuran,Xu, You. 2016

[4]Regeneration of Chinese cabbage transgenic plants expressing antibacterial peptide gene and cowpea trypsin inhibitor gene. Zhao, Junliang,Liang, Aihua,Zhu, Zhen,Tang, Yixiong. 2006

[5]Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. Jones, H. D.,Li, K. X.,He, Y.,Chen, S.,Chen, X. M.,Wang, D. S.,Xia, L. Q.,Wang, D. W.. 2010

[6]Research Progress on Transformation Maize Mediated by Agrobacterithm Tumefaciens. Li, Xiuping,Li, Xiuping,Jiang, Lijing,Liu, Na. 2011

[7]Agrobacterium tumefaciens-mediated transformation of poinsettia, Euphorbia pulcherrima, with virus-derived hairpin RNA constructs confers resistance to Poinsettia mosaic virus. Clarke, Jihong Liu,Spetz, Carl,Haugslien, Sissel,Xing, Shaochen,Dees, Merete W.,Blystad, Dag-Ragnar,Xing, Shaochen,Dees, Merete W.,Moe, Roar. 2008

[8]Expression of alfalfa antifungal peptide gene and enhance of resistance to Verticillium dahliae in upland cotton. Zhang, Haiping,Wang, Xuede,Shao, Mingyan,Yuan, Shuna,Ni, Mi,Zhang, Haiping.

[9]Agrobacterium-mediated multiple gene transformation in rice using a single vector. Cao, MX,Huang, JQ,Wei, ZM,Yao, QH,Wan, CZ,Lu, JA. 2005

[10]Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene. Sun, CB,Kong, QL,Xu, WS. 2002

[11]Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens. Tao Li-li,Yin Gui-xiang,Du Li-pu,Shi Zheng-yuan,She Mao-yun,Xu Hui-jun,Ye Xing-guo. 2011

[12]Study on transformation of cysteine proteinase inhibitor gene into cabbage (Brassica oleracea var. capitata L.). Lei, Jian Jun,Yuan, Song Hong,Yang, Wen Jie,Qiong, Li Cheng,Ying, Fu You. 2006

[13]Regeneration and production of transgenic Lilium longiflorum via Agrobacterium tumefaciens. Liu, Juhua,Zhang, Jing,Xu, Biyu,Jia, Caihong,Zhang, Jianbin,Tan, Guanglan,Jin, Zhiqiang.

[14]Efficient regeneration and transformation of Spathiphyllum cannifolium. Yu, Bo,Liao, Feixiong,Liu, Jinmei,Sun, Yingbo,Huang, Lili.

[15]Microscopic observations of strawberry plant colonization by a GFP-labelled strain of Fusarium oxysporum f. sp. fragariae. Yuan, Hongbo,Ling, Xitie,Liu, Tingli,Chen, Tianzi,Yang, Yuwen,Yao, Shu,Zhang, Baolong.

[16]Phosphomannose-isomerase (pmi) gene as a selectable marker for rice transformation via Agrobacterium. He, ZQ,Fu, YP,Si, HM,Hu, GC,Zhang, SH,Yu, YH,Sun, ZX.

[17]Purification and characterization of beta-glucosidase from newly isolated Aspergillus sp MT-0204. Qi, Bin,Liu, Xianjin,Qi, Bin,Wang, Limei. 2009

[18]Influence of the application of three different elicitors on soybean plants on the concentrations of several isoflavones in soybean seeds. Zhang, Bo,Hettiarachchy, Navam,Chen, Pengyin,Horax, Ronny,Cornelious, Brian,Zhu, Danhua.

[19]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[20]Light effect on the tissue contents and distribution of isoflavones in the developing seedling of soybean. Sun, JM,Ding, AL. 1998

作者其他论文 更多>>