Small interfering RNA targeting nonstructural protein1 alpha (nsp1 alpha) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells

文献类型: 外文期刊

第一作者: Shi, Xibao

作者: Shi, Xibao;Zhang, Xiaozhuan;Li, Chunxi;Shi, Xibao;Chang, Yongzhe;Zhang, Gaiping;Shi, Xibao;Chang, Yongzhe;Wang, Li;Chen, Jing;Wang, Chao;Deng, Ruiguang;Zhang, Gaiping;Zhang, Xiaozhuan;Jiang, Kai;Fan, Jianming

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关键词: PRRSV;Small interfering RNA;nsp1 alpha

期刊名称:RESEARCH IN VETERINARY SCIENCE ( 影响因子:2.534; 五年影响因子:2.382 )

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收录情况: SCI

摘要: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strategy against viral infection. Previous studies have indicated that the nonstructural protein 1 alpha (nsp1 alpha) of PRRSV may take an important role in virulence of PRRSV. The present work was involved to explore the effect of siRNA targeting nsp1 alpha on the replication of PRRSV in MARC-145 cells, and the results showed that over-expression of nsp1 alpha enhanced the replication of PRRSV and that siRNAs specifically targeting nsp1 alpha significantly inhibited the replication of PRRSV in MARC-145 cells. In conclusion, this work indicated that nsp1 alpha may be a viral pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1 alpha may be a new strategy to control PRRSV in the future. (C) 2015 Elsevier Ltd. All rights reserved.

分类号: S85

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