A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector

文献类型： 外文期刊

第一作者： Li, Shuo

作者： Li, Shuo;Wang, Xi;Ji, Yinghua;Zhou, Yijun;Wang, Xi;Xu, Jianxiang

作者机构：

关键词： Rice stripe virus;Rice black-streaked dwarf virus;Simultaneous detection via RT-PCR

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two viruses in vector, which could facilitate better forecasting and control of the virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously. (C) 2014 Elsevier B.V. All rights reserved.

分类号： R37

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