A strain of Aspergillus flavus from China shows potential as a biocontrol agent for aflatoxin contamination

文献类型： 外文期刊

第一作者： Zhou, Lu

作者： Zhou, Lu;Wei, Dan-Dan;Selvaraj, Jonathan Nimal;Shang, Bo;Zhang, Chu-Shu;Xing, Fu-Guo;Zhao, Yue-Ju;Wang, Yan;Liu, Yang

作者机构：

关键词： aflatoxin;Aspergillus flavus;atoxigenic strain;aflatoxin gene cluster;biocontrol

期刊名称：BIOCONTROL SCIENCE AND TECHNOLOGY （ 影响因子：1.665； 五年影响因子：1.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Aflatoxins are toxic and carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus. Strains of A. flavus that are non-aflatoxigenic (i.e., incapable of secreting aflatoxins) have proven effective in controlling contamination by these aflatoxin producing species in the field. In the present study, a non-aflatoxigenic A. flavus strain, GD-3, was isolated from a peanut field in Guangdong Province, China. Polymerase chain reaction (PCR) analysis showed that 12 aflatoxin biosynthesis genes (aflT, pksA, nor-1, fas-2, fas-1, aflR, aflJ, adhA, estA, norA, ver-1 and verA) were deleted in GD-3. Co-inoculation with a toxigenic strain, GD-15, at the ratio of 1: 10, 1: 1 or 10: 1 (GD-3: GD-15), showed that GD-3 was capable of reducing detectable aflatoxin levels on three different substrates. This reduction ranged from 33% to 99% and correlated with competitor ratio. These results demonstrated that GD-3 was successful at reducing aflatoxin contamination and showed promise as a potential agent of biocontrol for local farmers.

分类号： S476

  • 相关文献

[1]Isolation and characterization of Aspergillus flavus strains in China. Mamo, Firew Tafesse,Wang, Yan,Liu, Yang,Mamo, Firew Tafesse,Wang, Yan,Liu, Yang,Shang, Bo,Selvaraj, Jonathan Nimal. 2018

[2]Aflatoxin B-1 inhibition in Aspergillus flavus by Aspergillus niger through down-regulating expression of major biosynthetic genes and AFB(1) degradation by atoxigenic A. flavus. Xing, Fuguo,Wang, Limin,Liu, Xiao,Selvaraj, Jonathan Nimal,Wang, Yan,Zhao, Yueju,Liu, Yang.

[3]Development and evaluation of ITS- and aflP-based LAMP assays for rapid detection of Aspergillus flavus in food samples. Liu, Peiqing,Li, Benjin,Yin, Rongmei,Weng, Qiyong,Chen, Qinghe.

[4]Effect of water activity and temperature on the growth of Aspergillus flavus, the expression of aflatoxin biosynthetic genes and aflatoxin production in shelled peanuts. Liu, Xiao,Guan, Xuanli,Xing, Fuguo,Lv, Cong,Dai, Xiaofeng,Liu, Yang.

[5]Ethylene inhibited aflatoxin biosynthesis is due to oxidative stress alleviation and related to glutathione redox state changes in Aspergillus flavus. Liao, B. -S.. 2009

[6]Inhibitory Effect of Cinnamaldehyde, Citral, and Eugenol on Aflatoxin Biosynthetic Gene Expression and Aflatoxin B-1 Biosynthesis in Aspergillus flavus. Liang, Dandan,Xing, Fuguo,Selvaraj, Jonathan Nimal,Liu, Xiao,Wang, Limin,Hua, Huijuan,Zhou, Lu,Zhao, Yueju,Wang, Yan,Liu, Yang.

[7]Optimization of Aflatoxin Production of Aspergillus Flavus on peanuts. Zhang Chushu,Sun Jie,Yu Lina,Yang Qingli,Feng Jianxiong. 2014

[8]Study on the key environmental factors Aspergillus flavus growth and aflatoxin production. Zhang Chushu,Sun Jie,Yu Lina,Bi Jie,Yang Qingli,Zhao Qin,Feng Jianxiong,Fu Xiaoji,Gao Yanan. 2014

[9]Gene Expression Profiling and Identification of Resistance Genes to Aspergillus flavus Infection in Peanut through EST and Microarray Strategies. Bhatnagar, Deepak,Yu, Jiujiang,Guo, Baozhu,Fedorova, Natalie D.,Wan, Chun-Hua,Wang, Wei,Nierman, William C.,Chen, Xiaoping,Chen, Xiaoping,Nierman, William C.. 2011

[10]Cinnamaldehyde inhibits fungal growth and aflatoxin B-1 biosynthesis by modulating the oxidative stress response of Aspergillus flavus. Sun, Qi,Shang, Bo,Liu, Yang,Wang, Ling,Lu, Zhisong.

[11]Autoxidated linolenic acid inhibits aflatoxin biosynthesis in Aspergillus flavus via oxylipin species. Yan, Shijuan,Chen, Zhuang,Yan, Shijuan,Liang, Yating,Zhang, Jindan,Liu, Chun-Ming.

[12]Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins. Li, Peiwu,Zhou, Qian,Wang, Ting,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Zhou, Qian,Chang, Perng-Kuang. 2016

[13]The effectiveness of ISSR profiling for studying genetic diversity of Aspergillus flavus from peanut-cropped soils in China. Zhang, Chu-Shu,Xing, Fu-Guo,Selvaraj, Jonathan Nimal,Zhou, Lu,Zhao, Yue-Ju,Liu, Yang,Zhang, Chu-Shu,Yang, Qing-Li.

[14]Molecular Characterization of Atoxigenic Aspergillus flavus Isolates Collected in China. Wei, Dandan,Zhou, Lu,Selvaraj, Jonathan Nimal,Zhang, Chushu,Xing, Fuguo,Zhao, Yueju,Wang, Yan,Liu, Yang. 2014

[15]Antifungal compounds from Bacillus subtilis B-FS06 inhibiting the growth of Aspergillus flavus. Zhang, Ting,Shi, Zhi-Qi,Wang, Fei,Zhang, Ting,Hu, Liang-Bin,Cheng, Luo-Gen.

[16]Transcriptome identification of the resistance-associated genes (RAGs) to Aspergillus flavus infection in pre-harvested peanut (Arachis hypogaea). Chen, Xiao-Ping,Li, Hai-Fen,Liu, Hai-Yan,Hong, Yan-Bin,Liang, Xuan-Qiang,Wang, Tong,Li, Ling,Liang, Xuan-Qiang,Wang, Tong,Yang, Qing-Li,Chi, Xiao-Yuan,Yang, Zhen,Yu, Shan-Lin. 2013

[17]Effect of ozone on aflatoxins detoxification and nutritional quality of peanuts. Chen, Ran,Ma, Fei,Li, Pei-Wu,Zhang, Wen,Ding, Xiao-Xia,Zhang, Qi,Li, Min,Wang, Yan-Ru,Xu, Bao-Cheng,Chen, Ran,Li, Pei-Wu,Zhang, Wen,Li, Min,Wang, Yan-Ru,Xu, Bao-Cheng,Ma, Fei,Li, Pei-Wu,Zhang, Qi,Chen, Ran,Ma, Fei,Li, Pei-Wu,Ding, Xiao-Xia,Zhang, Qi,Li, Min,Wang, Yan-Ru,Xu, Bao-Cheng,Li, Pei-Wu,Zhang, Wen,Ding, Xiao-Xia.

[18]Ultrasensitive nanogold probe-based immunochromatographic assay for simultaneous detection of total aflatoxins in peanuts. Zhang, Daohong,Li, Peiwu,Zhang, Qi,Zhang, Wen,Zhang, Daohong,Li, Peiwu,Zhang, Wen,Zhang, Daohong,Li, Peiwu,Zhang, Wen.

[19]Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS). Busman, Mark,Bobell, John R.,Maragos, Chris M.,Liu, Jihong,Zhong, Hongjian. 2014

[20]Immunoassays for aflatoxins. Li, Peiwu,Zhang, Qi,Zhang, Wen.

作者其他论文 更多>>

微信小程序