Fabrication of a polystyrene microfluidic chip coupled to electrospray ionization mass spectrometry for protein analysis

文献类型： 外文期刊

第一作者： Hu, Xianqiao

作者： Hu, Xianqiao;Zhu, Zhiwei;Hu, Xianqiao;Dong, Yuanyuan;He, Qiaohong;Chen, Hengwu;Hu, Xianqiao;Zhu, Zhiwei

作者机构：

关键词： Microfluidic chip;Electrospray ionization-mass spectrometry;Polystyrene;Immobilized enzymatic reactor;Gold film electric contact;Interface

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

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收录情况： SCI

摘要： A highly integrated polystyrene (PS) microfluidic chip coupled to electrospray ionization mass spectrometry for on-chip protein digestion and online analysis was developed. The immobilized enzymatic microreactor for on-chip protein digestion was integrated onto microchip via the novel method of region-selective UV-modification combined with glutaraldehyde-based immobilization. The micro film electric contact for applying high voltage was prepared on chips by using UV-directed electroless plating technique. A micro-tip was machined at the end of main channel, serving as the interface between microchip and mass spectrometric detector. On-chip digestion and online detection of protein was carried out by coupling the microchip with mass spectrometry (MS). The influences of methanol flow rate in side channel on the stability of spray and intensity of signals were investigated systematically. Also the influence of sample flow rate on the performance of immobilized enzymatic reactor were investigated. Stable spray was obtained at the spray voltage of 2.8-3.0 kV and the methanol flow rate of 500-700 nL min(-1) with the relative standard deviation (RSD) of total ion current (TIC) less than 10%. The influence of sample flow rate on the performance of immobilized enzymatic reactor was also studied. The sequence coverage of protein identification decreased with the increase of flow rate of the sample solution. A sequence coverage of 96% was obtained with immobilized enzymatic reactor at the sample flow rate of 100 nL min(-1) with the reaction time of 8.4 min. It could detect cytochrome c as low as 10 mu g mL(-1) with the developed system. No obvious decrease in protein digestion efficiency was observed after the chip continuously performed for 4 h and stored for 15 d. (C) 2015 Elsevier B.V. All rights reserved.

分类号： Q503

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