The M949_1556 gene plays a role on the bacterial antigenicity and pathogenicity of Riemerella anatipestifer

文献类型： 外文期刊

第一作者： Zou, Jiechi

作者： Zou, Jiechi;Wang, Xiaolan;Tian, Mingxing;Cao, Shoulin;Hou, Wanwan;Wang, Shaohui;Han, Xiangan;Ding, Chan;Yu, Shengqing

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关键词： Riemerella anatipestifer;Antigenicity;Pathogenicity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： Riemerella anatipestifer is one of the most economically important pathogens of farm ducks worldwide. However, the molecular mechanisms regarding its antigenicity and pathogenicity are poorly understood. We previously constructed a library of random Tn4351 transposon mutants using R. anatipesnfer strain CH3. In this study, M949_1556 gene inactivated mutant strain CH3 Delta M949_1556 was identified by screening of the library using monoclonal antibody against R. anatipeshfer serotype 1 lipopolysaccharide (LPS) (anti-LPS MAb) followed by sequence analysis. The mutant strain presented no reactivity to the anti-LPS MAb in an indirect ELISA. Animal studies showed that the median lethal dose (LD50) of CH3 Delta M949_1556 was >10(10) colony forming units (CFU), which was attenuated more than 50 times, compared with that of wild-type strain CH3 (LD50 = 2 x 10(8) CFU). The bacterial loads in the blood of CH3 Delta M949_1556 infected ducks were significantly decreased, compared with those of CH3-infected ducks. In addition, CH3 Delta M949_1556 presented significant, higher susceptibility to complement-dependent killing than CH3 did in vitro. Furthermore, CH3 Delta M949_1556 showed increased bacterial adhesion and invasion capacities to Vero cells. Immunization with CH3 Delta M949_1556-inactived vaccine was effective in protecting the ducks from challenge with R. anatipestifer serotype 1 strain WJ4, serotype 2 strain Yb2 and serotype 10 strain HXb2, suggesting that the mutant strain CH3 Delta M949_1556 could provide a broad cross-protection among R. anatipeshfer serotypes 1,2 and 10 strains. Our results demonstrated that the M949_1556 gene plays a role on the bacterial antigenicity and pathogenicity of R. anatipestifer. (C) 2015 Elsevier B.V. All rights reserved.

分类号： S8

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