GLUCAN SYNTHASE-LIKE 5 (GSL5) Plays an Essential Role in Male Fertility by Regulating Callose Metabolism During Microsporogenesis in Rice

文献类型： 外文期刊

第一作者： Shi, Xiao

作者： Shi, Xiao;Sun, Xuehui;Zhang, Zhiguo;Feng, Dan;Zhang, Qian;Han, Lida;Wu, Jinxia;Lu, Tiegang

作者机构：

关键词： Callose;Male fertility;Microspore development;Pollen viability;Rice (Oryza sativa)

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

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收录情况： SCI

摘要： Callose plays an important role in pollen development in flowering plants. In rice, 10 genes encoding putative callose synthases have been identified; however, none of them has been functionally characterized. In this study, a rice Glucan Synthase-Like 5 (GSL5) knock-out mutant was isolated that exhibited a severe reduction in fertility. Pollen viability tests indicated that the pollen of the mutant was abnormal while the embryo sac was normal. Further, GSL5-RNA interference transgenic plants phenocopied the gsl5 mutant. The RNA expression of GSL5 was found to be knocked out in the gsl5 mutant and knocked down in GSL5-RNA interference transgenic plants by real-time reverse transcripion-PCR (RT-PCR) analysis. The male sterility of the mutant was due to abnormal microspore development; an analysis of paraffin sections of the mutant anthers at various developmental stages revealed that abnormal microspore development began in late meiosis. Both the knock-out and knock-down of GSL5 caused a lack of callose in the primary cell wall of meiocytes and in the cell plate of tetrads. As a result, the callose wall of the microspores was defective. This was demonstrated by aniline blue staining and an immunogold labeling assay; the microspores could not maintain their shape, leading to premature swelling and even collapsed microspores. These data suggest that the callose synthase encoded by GSL5 plays a vital role in microspore development during late meiosis and is essential for male fertility in rice.

分类号： Q945

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