An aptamer-based signal-on bio-assay for sensitive and selective detection of Kanamycin A by using gold nanoparticles

文献类型: 外文期刊

第一作者: Chen, Jing

作者: Chen, Jing;Li, Zhaohui;Ge, Jia;Yang, Ran;Zhang, Lin;Qu, Ling-bo;Qu, Ling-bo;Wang, Hong-qi;Zhang, Ling

作者机构:

关键词: Aptamer;Fluorescence;Gold nanoparticles;Kanamycin A

期刊名称:TALANTA ( 影响因子:6.057; 五年影响因子:5.386 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In this study, a simple and sensitive aptamer-based fluorescence method for the detection of Kanamycin A by using gold nanoparticles (AuNPs) has been developed. In this assay, AuNPs were utilized as DNA nanocarrier as well as efficient fluorescence quencher. In the absence of Kanamycin A, dye-labeled aptamer could be adsorbed onto the surface of AuNPs and the fluorescence signal was quenched. In the presence of Kanamycin A, the specific binding between dye-labeled aptamer and its target induced the formation of rigid structure, which led to dye-labeled aptamer releasing from the surface of AuNPs and the fluorescence intensity was recovered consequently. Under optimum conditions, calibration modeling showed that the analytical linear range covered from 0.8 nM to 350 nM and the detection limit of 0.3 nM was realized successfully. This proposed bio-assay also showed high selectivity over other antibiotics. Meanwhile, this strategy was further used to determine the concentrations of Kanamycin A in milk sample with satisfying results. (C) 2015 Elsevier B.V. All rights reserved.

分类号: O65

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