Comparative analysis of selected innate immune-related genes following infection of immortal DF-1 cells with highly pathogenic (H5N1) and low pathogenic (H9N2) avian influenza viruses

文献类型： 外文期刊

第一作者： Liu, Ai-ling

作者： Liu, Ai-ling;Pan, Jie;Li, Yu-feng;Ma, Xiu-Li;Yu, Ke-xiang;Huang, Bing;Song, Min-xun;Qi, Wenbao;Liao, Ming;Li, Feng;Li, Feng

作者机构：

关键词： H5N1;H9N2;Innate immune-related genes;Real-time quantitative PCR;Subtype-dependent host response

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： H5N1 and H9N2 viruses are important causes of avian influenza in China. H5N1 is typically associated with severe to fatal disease in poultry, while H9N2 is usually associated with mild disease. Differences in viral virulence prompted us to investigate whether innate immune responses would be differentially regulated following infection by H5N1 and H9N2 viruses. To address this hypothesis, expression of a panel of innate immune-related genes including IFN-alpha, IFN-beta, Mx1, OASL, ISG12, IFIT5, IRF7, USP18, SST, and KHSRP in immortal DF-1 cells following H5N1 and H9N2 infection was analyzed and compared by real-time quantitative RT-PCR. Cells infected by either virus overall exhibited a similar expression profile for four ISGs (Mx1, OASL, ISG12, and IFIT5), IFN-alpha, IFN-beta, and SST gene. However, two immune-regulatory genes (IRF7 and KHSRP) were not responsive to highly pathogenic H5N1 infection but were strongly up-regulated in DF-1 cells infected with low pathogenic H9N2 infection. The subtype-dependent host response observed in this study offers new insights into the potential roles of IRF7 and KHSRP in control and modulation of the replication and virulence of different subtypes or strains of avian influenza A virus.

分类号： R37

  • 相关文献

[1]Generation and application of replication-competent Venus-expressing H5N1, H7N9, and H9N2 influenza A viruses. Wang, Guangwen,Zhang, Jie,Kong, Fandi,Li, Qibing,Wang, Jinliang,Ma, Shujie,Zhao, Yuhui,Liang, Libin,Li, Junping,Sun, Nan,Guan, Lizheng,Zhou, Yuan,Zhou, Chenchen,Huang, Shanyu,Bu, Zhigao,Jiang, Li,Chen, Hualan,Li, Chengjun. 2018

[2]Diversified reassortant H9N2 avian influenza viruses in chicken flocks in northern and eastern China. Huang, Yanyan,Hu, Beixia,Zhang, Xiumei,Huang, Yanyan,Wen, Xintian,Cao, Sanjie,Du, Qijing,Gavrilov, Boris K.,Khan, Mazhar I.. 2010

[3]Novel genetic reassortants in H9N2 influenza A viruses and their diverse pathogenicity to mice. Bi, Yuhai,Lu, Lu,Li, Jing,Zeshan, Basit,Sun, Lei,Liu, Wenjun,Lu, Lu,Yin, Yanbo,Qin, Zhuoming,Zhang, Yi,Gao, Huijie,Liu, Jinhua. 2011

[4]Recombinant Newcastle disease virus expressing H9 HA protects chickens against heterologous avian influenza H9N2 virus challenge. Nagy, Abdou,Lee, Jinhwa,Henningson, Jamie,Li, Yuhao,Ma, Jingjiao,Duff, Michael,Li, Yonghai,Lang, Yuekun,Yang, Jianmei,Richt, Juergen,Ma, Wenjun,Nagy, Abdou,Abdallah, Fatma,Ali, Ahmed,Mena, Ignacio,Garcia-Sastre, Adolfo,Mena, Ignacio,Garcia-Sastre, Adolfo,Yang, Jianmei,Garcia-Sastre, Adolfo.

[5]Evolution of H9N2 influenza viruses from domestic poultry in Mainland China. Li, CJ,Yu, KZ,Tian, GB,Yu, DD,Liu, LL,Jing, B,Ping, JH,Chen, HL.

[6]Lowly pathogenic avian influenza (H9N2) infection in Plateau pika (Ochotona curzoniae), Qinghai Lake, China. Yu, Zhijun,Zhang, Kun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Zhang, Kun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Cheng, Kaihui,Sun, Weiyang,Xin, Yue,Li, Lin,Huang, Jing,Sang, Xiaoyu,Li, Xue,Wang, Tiecheng,Qian, Jun,Gao, Yuwei,Xia, Xianzhu,Cai, Jinshan,Ma, Ruilin,Zhao, Quanbang,Cheng, Kaihui,Huang, Jing,Li, Xue.

[7]Influenza virus-like particles harboring H9N2 HA and NA proteins induce a protective immune response in chicken. Li, Xin,Ju, Houbin,Liu, Jian,Yang, Dequan,Qi, Xinyong,Yang, Xianchao,Ge, Feifei,Zhou, Jinping,Qiu, Yafeng,Zheng, Jie. 2017

[8]Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds:High Homology of Internal Genes with Human H10N8 Virus. Ye, Ge,Liang, Chai Hong,Ping, Hua Yu,Hua, Deng Guo,Lan, Chen Hua,Song, Lei Yong,Xiang, Yang Guo,Guang, Chen. 2016

[9]Muscovy duck retinoic acid-induced gene I (MdRIG-I) functions in innate immunity against H9N2 avian influenza viruses (AIV) infections. Cheng, Yuqiang,Huang, Qingqing,Ji, Wenhui,Du, Bin,Fu, Qiang,An, Huiting,Wang, Hengan,Yan, Yaxian,Ding, Chan,Sun, Jianhe,Ding, Chan,Li, Jing.

[10]N-glycan profiles in H9N2 avian influenza viruses from chicken eggs and human embryonic lung fibroblast cells. Chen, Wentian,Zhong, Yaogang,Su, Rui,Qi, Huicai,Deng, Weina,Sun, Yu,Ma, Tianran,Wang, Xilong,Yu, Hanjie,Li, Zheng,Wang, Xiurong.

[11]Two Genetically Similar H9N2 Influenza A Viruses Show Different Pathogenicity in Mice. Liu, Qingtao,Liu, Yuzhuo,Yang, Jing,Huang, Xinmei,Han, Kaikai,Zhao, Dongmin,Bi, Keran,Li, Yin,Liu, Qingtao,Liu, Yuzhuo,Yang, Jing,Huang, Xinmei,Han, Kaikai,Zhao, Dongmin,Bi, Keran,Li, Yin. 2016

[12]Characterization of influenza A virus with nine segments: Effect gene segment on virus property. Wu, Rui,Shao, Huabin,Luo, Qingping,Yang, Jun,Xu, Diping,Wu, Rui,Shao, Huabin,Xu, Diping,Wu, Rui,Zhang, Xuke. 2012

[13]Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.). Wang, Chuan Tang,Wang, Xiu Zhen,Chi, Xiao Yuan,Wu, Qi,Chen, Dian Xu,Tang, Yue Yi,Yang, Guan Pin,Feng, Tong,Gao, Hua Yuan,Xu, Ya Long. 2011

[14]Different Tolerance in Bread Wheat, Durum Wheat and Barley to Fusarium Crown Rot Disease Caused by Fusarium pseudograminearum. Liu, Yaxi,Wei, Yuming,Zheng, Youliang,Liu, Yaxi,Ma, Jun,Yan, Wei,Liu, Chunji,Ma, Jun,Yan, Guijun,Yan, Wei,Zhou, Meixue,Zhou, Meixue. 2012

[15]H5N1 avian influenza in China. Chen HuaLan,Chen HuaLan. 2009

[16]Analysis on Differentially Expressed Genes of Muscle Tissues in Rugao Chicken at the Ages of 2 and 12 Weeks by Microarray. Luan, De Qin,Bin Chang, Guo,Zhen, Ting,Huang, Zheng Yang,Zhou, Wei,Chen, Guo Hong,Liu, Yan. 2011

[17]Molecular identification and interaction assay of the gene (OsUbc13) encoding a ubiquitin-conjugating enzyme in rice. Wang, Ya,Xu, Meng-yun,Liu, Jian-ping,Wang, Mu-gui,Tu, Ju-min,Wang, Ya,Yin, Hai-qing. 2014

[18]Correlation between polymerase activity and pathogenicity in two duck H5N1 influenza viruses suggests that the polymerase contributes to pathogenicity. Leung, Bo Wah,Brownlee, George G.,Chen, Hualan. 2010

[19]A subunit vaccine candidate derived from a classic H5N1 avian influenza virus in China protects fowls and BALB/c mice from lethal challenge. Liu, Guangliang,Zhang, Fangfang,Shi, Jianzhong,Tian, Guobing,Chen, Hualan,Yu, Kangzheng,Meng, Qingwen. 2013

[20]Identification of a trypsin gene from Scylla paramamosain and its expression profiling during larval development. Jiang, Keji,Zhang, Fengying,Zhang, Dan,Zhang, Yong,Qiao, Zhenguo,Ma, Lingbo,Tao, Qichang,Pi, Yan. 2011

作者其他论文 更多>>

微信小程序