Validation of reference genes for quantitative real-time PCR during latex regeneration in rubber tree

文献类型： 外文期刊

第一作者： Long, Xiangyu

作者： Long, Xiangyu;He, Bin;Gao, Xinsheng;Qin, Yunxia;Yang, Jianghua;Fang, Yongjun;Qi, Jiyan;Tang, Chaorong;He, Bin

作者机构：

关键词： Hevea brasiliensis;Reference genes;Latex regeneration;Algorithms;Quantitative real-time PCR

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： In rubber tree, latex regeneration is one of the decisive factors influencing the rubber yield, although its molecular regulation is not well known. Quantitative real-time PCR (qPCR) is a popular and powerful tool used to understand the molecular mechanisms of latex regeneration. However, the suitable reference genes required for qPCR are not available to investigate the expressions of target genes during latex regeneration. In this study, 20 candidate reference genes were selected and evaluated for their expression stability across the samples during the process of latex regeneration. All reference genes showed a relatively wide range of the threshold cycle values, and their stability was validated by four different algorithms (comparative delta Ct method, Bestkeeper, NormFinder and GeNorm). Three softwares (comparative delta Ct method, NormFinder and GeNorm) exported similar results that identify UBC4, ADF, UBC2a, eIF2 and ADF4 as the top five suitable references, and 18S as the least suitable one. The application of the screened references would improve accuracy and reliability of gene expression analysis in latex regeneration experiments. (C) 2015 Elsevier B.V. All rights reserved.

分类号： R394

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