文献类型: 外文期刊
第一作者: Cheng, Yuqiang
作者: Cheng, Yuqiang;Wang, Hengan;Shi, Shuduan;Yan, Yaxian;Sun, Jianhe;Sun, Yingjie;Ding, Chan;Li, Jing
作者机构:
关键词: duck;innate immune response;myeloid differentiation factor 88;Newcastle disease virus;Toll-like receptor 5
期刊名称:JOURNAL OF VETERINARY SCIENCE ( 影响因子:1.672; 五年影响因子:1.904 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2'-5'-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the bursa of Fabricius, spleen, trachea, lung, jejunum, rectum, and skin; moderately expressed in the muscular and glandular tissues, duodenum, ileum, caecurn, and pancreas; and minimally expressed in the heart, liver, kidney, and muscle. DF-1 or HeLa cells transfected with DNA constructs encoding dTLR5 can activate NF-kappa B leading to the activation of interleukin-6 (IL-6) promoter. When we challenged ducks with a Herts33 Newcastle disease virus (NDV), mRNA transcription of the antiviral molecules Mx, Double stranded RNA activated protein kinase (PKR), and OAS was up-regulated in the liver, lung, and spleen 1 and 2 days post-inoculation.
分类号: S8
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