Odorant-binding proteins display high affinities for behavioral attractants and repellents in the natural predator Chrysopa pallens

文献类型： 外文期刊

第一作者： Zhao-Qun Li

作者： Zhao-Qun Li;Shuai Zhang;Jun-Yu Luo;Si-BaoWang;Shuang-Lin Dong;Jin-Jie Cui

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关键词： Behavioral trial;Chrysopa pollens;Fluorescence binding assay;Odorant-binding protein

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY （ 影响因子：2.32； 五年影响因子：2.465 ）

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收录情况： SCI

摘要： Chrysopa pallens is an important natural predator of various pests in many different cropping systems. Understanding the sophisticated olfactory system of insect antennae is crucial for studying the physiological bases of olfaction and could also help enhance the effectiveness of C. pallens in biological control. However, functional studies of the olfactory genes in C. pollens are still lacking. In this study, we cloned five odorant-binding protein (OBP) genes from C. pollens (CpalOBPs). Quantitative RT-PCR results indicated that the five CpalOBPs had different tissue expression profiles. Ligand-binding assays showed that farnesol, farnesene, cis-3-hexenyl hexanoate, geranylacetone, beta-ionone, octyl aldehyde, decanal, nerolidol (Ki < 20 mu M), and especially 2-pentadecanone (Ki = 1.19 mu M) and 2-hexyl-1-decanol (Ki = 0.37 mu M) strongly bound to CpalOBP2. CpalOBP15 exhibited high binding affinities for beta-ionone, 2-tridecanone, trans-nerolidol, and dodecyl aldehyde. Behavioral trials using the 14 compounds exhibiting high binding affinities for the CpalOBPs revealed that nine were able to elicit significant behavioral responses from C. pallens. Among them, farnesene and its corresponding alcohol, farnesol, elicited remarkable repellent behavioral responses from C. pallens. Our study provides several compounds that could be selected to develop slow-release agents that attract/repel C. pallens and to improve the search for strategies to eliminate insect pests. (C) 2015 Elsevier Inc. All rights reserved.

分类号： Q4`Q5`R33

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