Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R-phycoerythrin from red algae Bangia fusco-purpurea

文献类型： 外文期刊

第一作者： Wu, Qiang

作者： Wu, Qiang;Fu, Xiao-Ping;Sun, Le-Chang;Liu, Guang-Ming;Cao, Min-Jie;Cai, Qiu-Feng;Fu, Xiao-Ping;Zhang, Qian;Liu, Guang-Ming;Cao, Min-Jie;Cai, Qiu-Feng;Liu, Guang-Ming;Cao, Min-Jie;Cai, Qiu-Feng

作者机构：

关键词： Antioxidant activity;circular dichroism;gastrointestinal digestion;purification;red algae Bangia fusco-purpurea;R-phycoerythrin

期刊名称：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY （ 影响因子：3.713； 五年影响因子：3.408 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： R-phycoerythrin (R-PE) was purified from the red algae Bangia fusco-purpurea after 35-50% ammonium sulphate fractionation followed by ion-exchange column chromatography on DEAE-Sepharose, resulting in a purity (A(565)/A(280)) ratio of 5.1. The circular dichroism spectroscopy results suggested that the structure of R-PE is predominately helical. The antioxidant activity of R-PE was studied and revealed changes in conformation and antioxidant activity at different temperatures and pH values. After in vitro-simulated gastrointestinal (GI) digestion of R-PE, the scavenging activity of ABTS radical (EC50, 769.9gmL(-1)), DPPH radical (EC50, 421.9gmL(-1)), hydroxyl radical (EC50, 32.4gmL(-1)) and reducing power (A(700)=0.5, 625.8gmL(-1)) were measured. Gel filtration chromatography analysis showed that the molecular weight distribution of the final GI digest that still contained high antioxidant activity was <3kDa. Our present results indicate that digestion-resistant antioxidant peptides of R-PE may be obtained by in vitro GI proteinases degradation.

分类号： TS2

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