Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus

文献类型： 外文期刊

第一作者： Zhu, Yingqi

作者： Zhu, Yingqi;Li, Chuanfeng;Bi, Zhuangli;Chen, Zongyan;Meng, Chunchun;Ding, Chan;Liu, Guangqing;Zhu, Yingqi;Bi, Zhuangli;Wang, Gui-Jun

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关键词： Suicidal DNA vaccine;Novel duck reovirus;Sigma C gene

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

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收录情况： SCI

摘要： A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against novel duck reovirus (NDRV). The sigma C gene of NDRV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. After transfected into BHK-21 cells, the antigenicity of the sigma C protein was confirmed using an indirect immunofluorescence and Western blot assay. Immunogenicity was studied in ducklings. The results showed that NDRV-specific antibodies, neutralizing antibodies, IFN-gamma and IL-4 were well induced in ducklings. Furthermore, all of the ducklings were protected against challenge with wild-type NDRV. (C) 2015 Elsevier B.V. All rights reserved.

分类号： S85

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