Rapid Metabolite Discovery, Identification, and Accurate Comparison of the Stereoselective Metabolism of Metalaxyl in Rat Hepatic Microsomes

文献类型: 外文期刊

第一作者: Wang, Xinru

作者: Wang, Xinru;Zhang, Ping;Wang, Yao;Zhou, Zhigiang;Zhu, Wentao;Qiu, Jing;Xu, Peng

作者机构:

关键词: metabolic profile;LC-MS;rat hepatic microsome;stereoselective metabolism;metabolite identification

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Metabolite identification and quantitation impose great challenges on risk assessment of agrochemicals, as many metabolite standards are generally unavailable. In this study, metalaxyl metabolites were identified by time-of-flight mass spectrometry and semiquantified by triple quadrupole tandem mass spectrometry with self-prepared C-13-labeled metalaxyl metabolites as internal standards. Such methodology was employed to characterize the stereoselective metabolism of metalaxyl in rat hepatic microsomes successfully. Metabolites derived from hydroxylation, demethylation, and didemethylation were identified and semiquantified. The results indicated that (+)-S-metalaxyl eliminated preferentially as the enantiomer fraction was 0.32 after 60 min incubation. The amounts of hydroxymetalaxyl and demethylmetalaxyl derived from (-)-R-metalaxyl were 1.76 and 1.82 times higher than that of (+)-S-metalaxyl, whereas didemethylmetalaxyl derived from (+)-S-metalaxyl was 1.44 times larger than that from (-)-R-metalaxyl. This study highlights a new quantitation approach for stereoselective metabolism of chiral agrochemicals and provides more knowledge on metalaxyl risk assessment.

分类号: R15`S

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