Conserved and novel heat stress-responsive microRNAs were identified by deep sequencing in Saccharina japonica (Laminariales, Phaeophyta)

文献类型： 外文期刊

第一作者： Liu, Fuli

作者： Liu, Fuli;Wang, Wenjun;Sun, Xiutao;Liang, Zhourui;Wang, Feijiu

作者机构：

关键词： miRNA;q-PCR;RNA-Seq;sRNA

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.228； 五年影响因子：7.791 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： As a temperate-cold species, Saccharina japonica often suffers heat stress when it is transplanted to temperate and subtropical zones. Study the heat stress response and resistance mechanism of Saccharina is of great significance for understanding the acclimation to heat stress under domestication as well as for breeding new cultivars with heat stress resistance. In this study, we identified a set of heat stress-responsive miRNAs and analysed their regulation during the heat stress response. CO (control) and heat stress (HS) sRNA libraries were constructed and sequenced. Forty-nine known miRNAs and 75 novel miRNAs were identified, of which seven known and 25 novel miRNAs were expressed differentially under heat stress. Quantitative PCR of six selected miRNAs confirmed that these loci were responsive to heat stress. Thirty-nine and 712 genes were predicted to be targeted by the seven known miRNAs and 25 novel miRNAs, respectively. Gene function and pathway analyses showed that these genes probably play important roles in S.japonica heat stress tolerance. The miRNAs identified represent the first set of heat-responsive miRNAs identified from S.japonica, and their identification can help elucidate the heat stress response and resistance mechanisms in S.japonica.

分类号： Q948

  • 相关文献

[1]Uncovering Male Fertility Transition Responsive miRNA in a Wheat Photo-Thermosensitive Genic Male Sterile Line by Deep Sequencing and Degradome Analysis. Bai, Jian-Fang,Wang, Yu-Kun,Wang, Peng,Duan, Wen-Jing,Yuan, Shao-Hua,Sun, Hui,Yuan, Guo-Liang,Ma, Jing-Xiu,Wang, Na,Zhang, Feng-Ting,Zhang, Li-Ping,Zhao, Chang-Ping,Bai, Jian-Fang,Wang, Yu-Kun,Wang, Peng,Duan, Wen-Jing,Yuan, Shao-Hua,Sun, Hui,Yuan, Guo-Liang,Ma, Jing-Xiu,Wang, Na,Zhang, Feng-Ting,Zhang, Li-Ping,Zhao, Chang-Ping,Wang, Peng,Duan, Wen-Jing. 2017

[2]Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity. Miao, Xiangyang,Qingmiao Luo Xiaoyu Qin. 2015

[3]Small Antisense RNA RbIR Positively Regulates RuBisCo in Synechocystis sp PCC 6803. Hu, Jinlu,Li, Tianpei,Zhan, Jiao,Chen, Hui,He, Chenliu,Li, Tianpei,Xu, Wen,Wang, Qiang. 2017

[4]Ecogenomic survey of plant viruses infecting Tobacco by Next generation sequencing. Akinyemi, Ibukun A.,Qi, Shuishui,Wu, Qingfa,Wang, Fang,Zhou, Benguo. 2016

[5]Overexpression of potato miR482e enhanced plant sensitivity to Verticillium dahliae infection. Yang, Liu,Mu, Xiaoying,Liu, Chao,Cai, Jinghui,Shi, Ke,Zhu, Wenjiao,Yang, Qing,Yang, Liu. 2015

[6]Characterization of a Novel Polerovirus Infecting Maize in China. Jiang, Guangzhuang,Wu, Jianxiang,Qian, Yajuan,Zhou, Xueping,Jiang, Guangzhuang,Zhou, Xueping,Liu, Yong. 2016

[7]The polymorphisms of kappa-casein gene and their associations with milk production traits and expression analysis in Chinese Holstein cattle. Ju, Zhihua,Huang, Jinming,Li, Qiuling,Wang, Hongmei,Zhong, Jifeng,Wang, Changfa. 2011

[8]Genome-wide analysis of Water-stress-responsive microRNA expression profile in tobacco roots. Yin, Fuqiang,Gao, Jian,Qin, Cheng,Zhang, Zhiming,Shen, Yaou,Lin, Haijian,Pan, Guangtang,Yin, Fuqiang,Liu, Ming,Zhang, Wenyou,Xia, Mingzhong,Qin, Cheng,Yang, Aiguo,Luo, Chenggang.

[9]Proteomic analysis of the phenotype of the scaleless wings mutant in the silkworm, Bombyx mori. Shi, Xiao-Feng,Han, Bin,Li, Yi-Nu,Zhang, Zhi-Fang,Han, Bin,Yi, Yong-Zhu,Shen, Xing-Jia,Li, Xiao-Ming.

[10]Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR. Wang, Yanhua,Wang, Guangxiang,Wang, Meng,Zhang, Delin,Yin, Hong.

[11]Peanut (Arachis hypogaea L.) Omics and Biotechnology in China. Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Liu, Shuan-Tao. 2011

[12]Small RNA profiling and degradome analysis reveal regulation of microRNA in peanut embryogenesis and early pod development. Gao, Chao,Wang, Pengfei,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Hou, Lei,Zhang, Ye,Li, Changsheng,Wang, Xingjun,Wang, Xingjun,Ju, Zheng. 2017

[13]Expression profiles of miRNAs in Gossypium raimondii. Jun MA,Teng-long GUO,Qing-lian WANG,Kun-bo WANG,Run-run SUN,Bao-hong ZHANG. 2015

[14]Deep Sequencing Analysis of miRNA Expression in Breast Muscle of Fast-Growing and Slow-Growing Broilers. Ouyang, Hongjia,He, Xiaomei,Li, Guihuan,Xu, Haiping,Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,He, Xiaomei,Li, Guihuan,Xu, Haiping,Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,He, Xiaomei,Li, Guihuan,Xu, Haiping,Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan. 2015

[15]MicroRNAs associated with drought response in the pulse crop common bean (Phaseolus vulgaris L.). Wu, Jing,Wang, Lanfen,Wang, Shumin. 2017

[16]Roles of DCL4 and DCL3b in rice phased small RNA biogenesis. Song, Xianwei,Li, Pingchuan,Zhai, Jixian,Zhou, Ming,Ma, Lijia,Cao, Shouyun,Liu, Chunyan,Chu, Chengcai,Wang, Xiu-Jie,Cao, Xiaofeng,Song, Xianwei,Li, Pingchuan,Zhai, Jixian,Zhou, Ming,Ma, Lijia,Cao, Shouyun,Liu, Chunyan,Chu, Chengcai,Wang, Xiu-Jie,Cao, Xiaofeng,Song, Xianwei,Zhou, Ming,Li, Pingchuan,Zhai, Jixian,Jeong, Dong-Hoon,Nakano, Mayumi,Green, Pamela J.,Meyers, Blake C.,Li, Pingchuan,Zhai, Jixian,Jeong, Dong-Hoon,Nakano, Mayumi,Green, Pamela J.,Meyers, Blake C.,Liu, Bin. 2012

[17]Integration of lncRNA-miRNA-mRNA reveals novel insights into oviposition regulation in honey bees. Chen, Xiao,Chen, Chao,Shi, Wei,Liu, Zhiguang,Wang, Huihua,Guo, Haikun,Ma, Ce,Lu, Qian. 2017

[18]Dissecting the biological relationship between TCGA miRNA and mRNA sequencing data using MMiRNA-Viewer. Bai, Yongsheng,Ding, Lizhong,Rath, Ethan,Stuart, Gary,Bai, Yongsheng,Stuart, Gary,Baker, Steve,Jiang, Feng,Bai, Jenny M.,Wu, Jianghong,Jiang, Hui. 2016

[19]The artificial microRNA mediates GUS-GFP gene silencing using ath-miR169d precursor as backbone. Liu, Chong,Zhang, Lan,Sun, Jie,Luo, Yanzhong,Fan, Yunliu,Wang, Lei,Liu, Chong,Sun, Jie,Wang, Mingbo. 2009

[20]Integrative RNA-and miRNA-Profile Analysis Reveals a Likely Role of BR and Auxin Signaling in Branch Angle Regulation of B. napus. Cheng, Hongtao,Hao, Mengyu,Wang, Wenxiang,Mei, Desheng,Liu, Jia,Wang, Hui,Sang, Shifei,Tang, Min,Zhou, Rijin,Chu, Wen,Fu, Li,Hu, Qiong,Wells, Rachel. 2017

作者其他论文 更多>>

微信小程序