Quality Evaluation of Five Commercial Enzyme Linked Immunosorbent Assay Kits for Detecting Aflatoxin B-1 in Feedstuffs

文献类型： 外文期刊

第一作者： Sun, Dan-dan

作者： Sun, Dan-dan;Gu, Xu;Li, Jun-guo;Yao, Ting;Dong, Ying-chao

作者机构：

关键词： Enzyme Linked Immunosorbent Assay Kit;Aflatoxin B-1;Evaluation;Feedstuffs

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The objective of this study was to evaluate the quality of five commercial enzyme linked immunosorbent assay (ELISA) kits (A, B, C, D, and E) from different suppliers for detecting afiatoxin B-1 (AFB(1)). AFB(1)-free corn samples supplemented with different levels of AFB(1) (5, 10, and 20 mu g/kg) were used as positive controls and 6 replicates of each control sample were tested to evaluate the accuracy and precision of these kits. In addition, we also evaluated the performance of these ELISA kits for AFB(1) in 30 feed samples, including corn, distillers dried grains with soluble, wheat samples, soybean meal, and poultry feed, which were verified by high performance liquid chromatography. Results showed that the coefficients of variation ranged from 1.18% to 16.22% in intra-plate and 2.85% to 18.04% in inter-plate for the determination of AFB(1). The half maximal inhibitory concentration for five kits ranged from 3.72 to 7.22 mu g/kg. The quantitation limits of AFB(1) were all under the legal limit in China but somewhat inconsistent with kit instructions. Although the recovery rate of four of the five kits were either less than 90% or more than 110%, all these values were acceptable in practice. Two kits had high false positive rates (C and E). In conclusion, our results revealed that the qualities of five tested ELISA kits were significantly different.

分类号： Q95

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