A novel angiotensin-I converting enzyme (ACE) inhibitory peptide from gastrointestinal protease hydrolysate of silkworm pupa (Bombyx mori) protein: Biochemical characterization and molecular docking study
文献类型: 外文期刊
第一作者: Wu, Qiongying
作者: Wu, Qiongying;Jia, Junqiang;Yan, Hui;Du, Jinjuan;Gui, Zhongzheng;Wu, Qiongying;Jia, Junqiang;Yan, Hui;Gui, Zhongzheng
作者机构:
关键词: Silkworm pupa (Bombyx mori);ACE inhibitory peptide;Gastrointestinal protease;Inhibition pattern;Molecular docking
期刊名称:PEPTIDES ( 影响因子:3.75; 五年影响因子:3.389 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Silkworm pupa (Bombyx mori) protein was hydrolyzed using gastrointestinal endopeptidases (pepsin, trypsin and alpha-chymotrypsin). Then, the hydrolysate was purified sequentially by ultrafiltration, gel filtration chromatography and RP-HPLC. A novel ACE inhibitory peptide, Ala-Ser-Leu, with the IC50 value of 102.15 mu M, was identified by IT-MS/MS. This is the first report of Ala-Ser-Leu from natural protein. Lineweaver-Burk plots suggest that the peptide is a competitive inhibitor against ACE. The molecular docking studies revealed that the ACE inhibition of Ala-Ser-Leu is mainly attributed to forming very strong hydrogen bonds with the S1 pocket (A1a354) and the S2 pocket (G1n281 and His353). The results indicate that silkworm pupa (B. mori) protein or its gastrointestinal protease hydrolysate could be used as a functional ingredient in auxiliary therapeutic foods against hypertension. (C) 2014 Elsevier Inc. All rights reserved.
分类号: Q5
- 相关文献
作者其他论文 更多>>
-
Fast identification of the BmNPV infected silkworms by portable NIR spectroscopy and chemometrics
作者:Liu, Yihan;Deng, Shuanglin;Li, Yurong;Zhang, Yeshun;Zhang, Guozheng;Yan, Hui;Li, Yurong;Zhang, Yeshun;Zhang, Guozheng;Yan, Hui;Li, Yurong;Zhang, Yeshun;Zhang, Guozheng;Yan, Hui
关键词:Portable; NIR spectroscopy; Silkworms; Virus; BmNPV; Identification
-
Macrophage β-arrestin-1 deteriorates DSS-induced colitis through interaction with NF-κB signaling
作者:Ke, Ping;Zhu, Dan-Ni;Liu, Meng-Zhen;Yan, Hui;Zhao, Qing-Jie;Liu, Chong;Ke, Ping;Du, Jing;Wei, Wei;Chen, Xiong-Wen;Chen, Xiong-Wen
关键词:beta-arrestin-1; Ulcerative colitis; Macrophage; NF-kappa B; Inflammation
-
A novel injectable sericin hydrogel with strong fluorescence for tracing
作者:Zhang, Yeshun;Cao, Xinyu;Zhang, Guozheng;Zhu, Mingkun;Yan, Hui;Li, Yurong;Zhang, Yeshun;Zhang, Guozheng;Zhu, Mingkun;Yan, Hui;Li, Yurong;Zhang, Yeshun;Zhang, Jingya
关键词:Sericin; Hydrogel; Self-luminescence; Double crosslinking
-
Effect of cordyceps militaris on growth performance, antioxidant capacity, and intestinal epithelium functions in weaned pigs
作者:Li, YanPing;Yu, Bing;Huang, Zhiqing;Luo, Yuheng;Zheng, Ping;Mao, Xiangbing;Yu, Jie;Luo, Junqiu;Yan, Hui;He, Jun;Li, YanPing;Yu, Bing;Huang, Zhiqing;Luo, Yuheng;Zheng, Ping;Mao, Xiangbing;Yu, Jie;Luo, Junqiu;Yan, Hui;He, Jun;Lu, Yang
关键词:antioxidative capacity; cordyceps militaris; growth performance; intestinal function; weaned pigs
-
Whole Genome Sequencing Analysis of Cellulose-degrading Bacterium DC11 Isolated from Silkworm Excrement and Characterization of Its Key Cellulase Gene ytoP Running Head: Characterization of strain DC11 and its key gene ytoP
作者:Zhang, Yuanhao;Li, Hao;Zhang, Minqi;Jiang, Xueping;Chen, Chen;Zhang, Ran;Gui, Zhongzheng;Li, Hao;Zhang, Ran;Gui, Zhongzheng;Zhang, Xiaohui;Huang, Gaiqun;Liu, Gang
关键词:Cellulose-degrading bacteria; Bacillus subtilis; Endoglucanase gene ytoP; Cloning; Expression
-
Comparative Analysis of Saccharification Characteristics of Different Type Sweetpotato Cultivars
作者:Li, Chen;Kou, Meng;Song, Weihan;Gao, Runfei;Tang, Wei;Yan, Hui;Wang, Xin;Zhang, Yungang;Li, Qiang;Arisha, Mohamed Hamed
关键词:Ipomoea batata; saccharification; maltose; starch; gelatinization temperature
-
Depletion of β-arrestin-1 in macrophages enhances atherosclerosis in ApoE-/- mice
作者:Shao, Bo-Zong;Liu, Meng-Zhen;Zhu, Dan-Ni;Yan, Hui;Ke, Ping;Han, Ting;Liu, Chong;Wei, Wei
关键词:beta-arrestin-1; Atherosclerosis; Autophagy; Macrophage; Inflammation