Determination of Brassinolide Analogs by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

文献类型： 外文期刊

第一作者： Yang, Jing

作者： Yang, Jing;Li, Xiuhuan;Huang, Qiliang;Wang, Dongwei

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关键词： Evaporative light scattering detector (ELSD);High-performance liquid chromatography (HPLC);Brassinolide analog

期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： An efficient method based on high-performance liquid chromatography with evaporative light scattering detection was developed for the separation and determination of four brassinolide analogs [24-epibrassinolide, (22S, 23S)-24-epibrassinolide, 28-homobrassinolide, and (22S, 23S)-28-epihomobrassinolide] without prior derivatization. The optimized analysis was carried out on a C-18 reversed-phase column (150mmx4.60mm, 3 mu m) at 30 degrees C using isocratic elution of acetonitrile and water (38:62, v/v). The drift tube temperature of the detector was 60 degrees C and the auxiliary gas (nitrogen) pressure was 360kPa. The regression equations revealed linear relationships (R-2=0.9984-0.9994) within the test ranges. The limits of detection and quantification were in the ranges of 0.12 to 0.17 mu g and 0.24 to 0.33 mu g, respectively. The fully validated method was applied to quantify the active ingredient content in technical material and formulations and provides an alternative approach for quality control.

分类号： O65

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