Improving furfural tolerance of Zymomonas mobilis by rewiring a sigma factor RpoD protein

文献类型： 外文期刊

第一作者： Tan, Fu-Rong

作者： Tan, Fu-Rong;Dai, Li-Chun;Wu, Bo;Qin, Han;Shui, Zong-Xia;Wang, Jing-Li;Zhu, Qi-Li;Hu, Qi-Chun;He, Ming-Xiong;Hu, Qi-Chun;He, Ming-Xiong;Ruan, Zhi-Yong

作者机构：

关键词： Furfural tolerance;Ethanol production;Global transcription machinery engineering;Error-prone PCR;Sigma factor;RpoD protein

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Furfural from lignocellulosic hydrolysates is the key inhibitor for bio-ethanol fermentation. In this study, we report a strategy of improving the furfural tolerance in Zymomonas mobilis on the transcriptional level by engineering its global transcription sigma factor (sigma(70), RpoD) protein. Three furfural tolerance RpoD mutants (ZM4-MF1, ZM4-MF2, and ZM4-MF3) were identified from error-prone PCR libraries. The best furfural-tolerance strain ZM4-MF2 reached to the maximal cell density (OD600) about 2.0 after approximately 30 h, while control strain ZM4-rpoD reached its highest cell density of about 1.3 under the same conditions. ZM4-MF2 also consumed glucose faster and yield higher ethanol; expression levels and key Entner-Doudoroff (ED) pathway enzymatic activities were also compared to control strain under furfural stress condition. Our results suggest that global transcription machinery engineering could potentially be used to improve stress tolerance and ethanol production in Z. mobilis.

分类号： Q939.9`Q81

  • 相关文献

[1]Complete mitogenome of the high ethanol production fungus Fusarium oxysporum Mh2-2. Zeng, Lu,Kang, Xincong,Xiong, Xingyao,Liu, Chichuan,Lin, Runmao,Xie, Bingyan,Xiong, Xingyao,Lin, Runmao. 2017

[2]Expression of mucoid induction factor MucE is dependent upon the alternate sigma factor AlgU in Pseudomonas aeruginosa. Yin, Yeshi,Damron, F. Heath,Withers, T. Ryan,Yu, Hongwei D.,Yu, Hongwei D.,Yin, Yeshi,Wang, Xin,Yu, Hongwei D.,Pritchett, Christopher L.,Schurr, Michael J.,Pritchett, Christopher L.,Damron, F. Heath. 2013

[3]Alternative sigma factor RpoN2 is required for flagellar motility and full virulence of Xanthomonas oryzae pv. oryzae. Yu, Chao,Li, Haiyun,Wu, Xiaoli,Li, Bo,Chen, Huamin,Wu, Maosen,He, Chenyang.

作者其他论文 更多>>

微信小程序