A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples
文献类型: 外文期刊
第一作者: Fu, Xiaoxiang
作者: Fu, Xiaoxiang;Wang, Xiaohan;Cui, Yongliang;Wang, Ali;Lai, Daowan;Wang, Baomin;Zhou, Ligang;Liu, Yang;Li, Qing X.
作者机构:
关键词: Ustiloxin A;icELISA;Mycotoxin;Phytotoxin;Rice false smut;Villosiclava virens
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Ustiloxin A, a cyclopeptide mycotoxin, was isolated from the pathogenic fungus Villosiclava virens that causes rice false smut, a worldwide devastating rice disease. A monoclonal antibody (mAb) 2D3G5 was generated with ustiloxin A-bovine serum albumin conjugate. A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) was then developed. It possessed a median inhibition concentration (IC50) of 13.8 ng/mL and a working range of 2.8-72 ng/mL. The mAb 2D3G5 recognized ustiloxin B with the cross-reactivity as 4%. The average recoveries of ustiloxin A from rice false smut balls and peeled rice samples ranged from 92% to 117% and from 92% to 107%, respectively. Comparison of the concentrations of ustiloxin A in rice false smut balls detected by both icELISA and high performance liquid chromatography-photodiode array detection indicated that the developed icELISA was suitable for detection of ustiloxin A in rice food and feed samples. (C) 2015 Elsevier Ltd. All rights reserved.
分类号: TS2`TS201.2
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