Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice

文献类型： 外文期刊

第一作者： Du, Luping

作者： Du, Luping;Li, Bin;Xu, Xiangwei;Sun, Bing;Pang, Fengjiao;Wen, Libin;He, Kongwang;Du, Luping;Li, Bin;Xu, Xiangwei;Sun, Bing;Pang, Fengjiao;Wen, Libin;He, Kongwang;Du, Luping;Li, Bin;Xu, Xiangwei;Sun, Bing;Pang, Fengjiao;Wen, Libin;He, Kongwang;Du, Luping;Li, Bin;Xu, Xiangwei;Sun, Bing;Pang, Fengjiao;Wen, Libin;He, Kongwang;Du, Luping;Xu, Xiangwei;Sun, Bing;Pang, Fengjiao;Huang, Kehe

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期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating viral disease of pigs. Safer and more effective vaccines are urgently needed. In this study, a synthetic ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) was adsorbed onto poly(D, L-lactide-co-glycolide)/polyethylenimine (PLGA/PEI) nanoparticles. We prepared a PLGA-nanoparticle-adsorbed PRRSV DNA vaccine and a PEI-DNA complex. The results showed that these model vaccines could significantly enhance humoral and cellular immune responses when compared with the responses induced by pcDNA3.1-SynORF5, a plasmid construct for expression of PRRSV ORF5. PLGA-branched PEI nanoparticles induced the most efficient immune response. The delivery system and adjuvant provide new models for the development of vaccines against PRRSV.

分类号： R37

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