Ovary transcriptome profiling of Coilia nasus during spawning migration stages by Illumina sequencing

文献类型: 外文期刊

第一作者: Shi, Ying

作者: Shi, Ying;Wei, Qi-Wei;Duan, Jin-Rong;Zhou, Yan-Feng;Xu, Dong-Po;Zhang, Min-Ying;Liu, Kai;Fang, Di-An;Fang, Di-An

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关键词: Coilia nasus;Spawning migration;Ovary transcriptome;Illumina sequencing

期刊名称:MARINE GENOMICS ( 影响因子:1.71; 五年影响因子:1.969 )

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收录情况: SCI

摘要: Coilia nasus is an anadromous kind of small to moderate size fish species, and limited transcriptomics research has been performed. In the present study, we performed Illumina sequencing to produce a 22,996,612 clean reads representing with a total of 4,599,079,601 (4.5 Gb) nucleotides comprehensive transcript dataset for ovary of C. nasus. Over 20 million total reads were assembled into 63,141 unigenes, and 27,027 annotated genes were predicted by Blastx and ESTScan, respectively. Applying Blast analysis and functional annotation (e.g., GO, COG, Swissprot and KEGG), we have sampled an extensive and diverse expressed gene catalog for C. nasus representing a large proportion of the genes expressed in the ovary development process. This approach will assist in the discovery and annotation of novel genes that play key roles in anadromous fish spawning migration process. (C) 2015 Elsevier B.V. All rights reserved.

分类号: Q3

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