Analysis of alpha-amylase inhibitor from corni fructus by coupling magnetic cross-linked enzyme aggregates of alpha-amylase with HPLC-MS

文献类型: 外文期刊

第一作者: Cen, Yin

作者: Cen, Yin;Liu, Fang;Yu, Jingang;Jiang, Xinyu;Chen, Xiaoqing;Liu, Liangliang

作者机构:

关键词: alpha-Amylase;Corni fructus;Cross-linked enzyme aggregates;HPLC-MS;Magnetic

期刊名称:JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ( 影响因子:3.205; 五年影响因子:3.068 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: As a carrier-free immobilization strategy, magnetic cross-linked enzyme aggregates (MCLEAs) showed improved enzyme activity, stability and magnetic response. In this study, MCLEAs of a-amylase (MCLEAs amylase) was prepared under optimized conditions and characterized with scanning electron microscope and vibrating sample magnetometer. The prepared MCLEAs amylase showed an amorphous structure and the saturation magnetization was 33.5 emu/g, which was sufficient for magnetic separation. Then MCLEAs amylase coupled with high performance liquid chromatography mass spectrometry (HPLC MS) was utilized to screen and identify a-amylase inhibitors from ethyl acetate extract of corni fructus. The experiment conditions were optimized. At the optimum conditions (incubation time: 10 min, pH: 7.0 and temperature: 20 degrees C), querciturone was successfully screened and identified with weak nonspecific binding. The screening result was verified by inhibition assays and the IC50 value of querciturone was 22.5 mu g/mL. This method provided a rapid way to screen active compounds from natural products. (C) 2015 Elsevier B.V. All rights reserved.

分类号: Q503

  • 相关文献

[1]Study on performance of magnetic fluorescent nanoparticles as gene carrier and location in pig kidney cells. Wang, Yan,Cui, Haixin,Sun, Changjiao,Du, Wei,Cui, Jinhui,Zhao, Xiang. 2013

[2]Determination of 5-hydroxymethyl-2-deoxycytidine in Rice by High-performance Liquid Chromatography-Tandem Mass Spectrometry with Isotope Dilution. Wang, Xiaoli,Wang, Shanshan,Chen, Xiangfeng,Chen, Yue,Yuan, Jinpeng,Zhao, Rusong,Guo, Tao. 2017

[3]Analysis of Xanthine Oxidase Inhibitors from Puerariae flos Using Centrifugal Ultrafiltration Coupled with HPLC-MS. Liangliang Liu,Li, Defang,Aiping Xiao,Lei Ma,Defang Li. 2017

[4]Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS. Liu, Liangliang,Leng, Juan,Yang, Xiai,Liao, Liping,Xiao, Aiping,Ma, Lei,Cen, Yin.

[5]Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum. Qiu, Jian,Luo, Shiqiao,Xiao, Xianzhou,Liu, Shizhong,Sun, Shuquan,Zhang, Jichuan,Zhang, Liqun,Wang, Feng,Liu, Shizhong. 2014

[6]Identification and Quantitation of Phenolic Compounds from the Seed and Pomace of Perilla frutescens Using HPLC/PDA and HPLC-ESI/QTOF/MS/MS. Guan, Zheng,Li, Sicong,Zhao, Yan,Liu, Jinchuan,Yang, Shuming,Chen, Ailiang,Lin, Zongtao,Yang, Ruinan. 2014

[7]A Survey of Naturally-Occurring Steroid Hormones in Raw Milk and the Associated Health Risks in Tangshan City, Hebei Province, China. Qu, Xueyin,Su, Chuanyou,Zheng, Nan,Li, Songli,Meng, Lu,Wang, Jiaqi,Qu, Xueyin,Su, Chuanyou,Zheng, Nan,Li, Songli,Meng, Lu,Wang, Jiaqi,Qu, Xueyin. 2018

[8]Methionine sulfone-containing orbitides, good indicators to evaluate oxidation process of flaxseed oil. Zou, Xian-Guo,Hu, Jiang-Ning,Zhu, Xue-Mei,Deng, Ze-Yuan,Zou, Xian-Guo,Hu, Jiang-Ning,Zhu, Xue-Mei,Deng, Ze-Yuan,Wang, Yu-Fu. 2018

[9]DETERMINATION OF LOTUS LEAF ALKALOIDS BY SOLID PHASE EXTRACTION COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY AND TANDEM MASS SPECTROMETRY DETECTION. Chen, Sha,Liu, Yanling,Li, Shaohua,Chen, Sha,Zhang, Haohao,Fang, Jinbao.

[10]Study on multiple pesticides by High Performance Liquid Chromatography-Mass Spectrometry using modified QuEChERS method - A case study on CHM. Dong, Yiwei,Bai, Wei,Li, Yuzhong,Xu, Chunying,Fang, Fuli,Guo, Zhicheng. 2012

[11]Comprehensive separation and identification of chemical constituents from Apocynum venetum leaves by high-performance counter-current chromatography and high performance liquid chromatography coupled with mass spectrometry. Zhang, Yuchi,Liu, Chunming,Wang, Jing,Wu, Guimei,Li, Sainan,Zhang, Zhengkun.

[12]Ability of resveratrol to inhibit advanced glycation end product formation and carbohydrate-hydrolyzing enzyme activity, and to conjugate methylglyoxal. Shen, Yixiao,Sheng, Zhanwu,Shen, Yixiao,Xu, Zhimin. 2017

[13]Screening, Gene Cloning, and Characterizations of an Acid-Stable alpha-Amylase. Liu, Xinyu,An, Yi,Cheng, Kun,Wang, Mingdao,Yang, Sen,Chen, Hongge,Jia, Wei.

[14]Isolation of -Amylase Inhibitors from Kadsura longipedunculata Using a High-Speed Counter-Current Chromatography Target Guided by Centrifugal Ultrafiltration with LC-MS. Cen, Yin,Chen, Xiaoqing,Xiao, Aiping,Liu, Liangliang.

[15]Improving the Catalytic Performance of a Talaromyces leycettanus alpha-Amylase by Changing the Linker Length. Zhang, Duoduo,Luo, Xuegang,Zhang, Tongcun,Zhang, Duoduo,Luo, Xuegang,Zhang, Tongcun,Tu, Tao,Wang, Yuan,Li, Yeqing,Zheng, Fei,Wang, Xiaoyu,Bai, Yingguo,Huang, Huoqing,Su, Xiaoyun,Yao, Bin,Luo, Huiying.

[16]Seed micromorphology and germination characteristics of wild and cultivated pepper strains. Zhang Zhuqing,Dai Xiongze,Zou Xuexiao,Ou Lijun,Ou Lijun.

[17]Phytosterols in banana (Musa spp.) flower inhibit alpha-glucosidase and alpha-amylase hydrolysations and glycation reaction. Sheng, Zhanwu,Pan, Siyi,Sheng, Zhanwu,Ai, Binling,Zheng, Lili,Zheng, Xiaoyan,Dai, Haofu,Prinyawiwatkul, Witoon,Xu, Zhimin.

[18]Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf. Liao, Liping,Chen, Jing,Liu, Liangliang,Xiao, Aiping. 2018

[19]Physicochemical characterization of enzymatically hydrolyzed heat treated granular starches. Mu, Tai-Hua,Abegunde, Oluwaseyi K.,Sun, Hong-Nan,Deng, Fu-Ming,Zhang, Miao.

[20]Overexpression of celB gene coding for beta-glucosidase from Pyrococcus furiosus using a baculovirus expression vector system in silkworm, Bombyx mori. Lin, Xu'Ai,Zhang, Wei,Chen, Yin,Bin Yao,Zhang, Zhi Fang.

作者其他论文 更多>>