Extraction of antibiotic zwittermicin A from Bacillus thuringiensis by macroporous resin and silica gel column chromatography
文献类型: 外文期刊
第一作者: Yan, Li
作者: Yan, Li;Liu, Jianguo;Li, Xia;Song, Fuping;Zhang, Jie
作者机构:
关键词: antibiotics;Bacillus thuringiensis;zwittermicin A;purification
期刊名称:BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY ( 影响因子:2.431; 五年影响因子:2.124 )
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年卷期:
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收录情况: SCI
摘要: To establish a production process capable of providing refined zwittermicin A (ZwA) on a large scale, the macroporous resin and silica gel column chromatography were used to separate and purify the antibiotic ZwA from the fermentation broth of Bacillus thuringiensis HD-1. The result of high-performance liquid chromatography-mass spectrometry after purification suggests that the samples of ZwA were of high purity, 89%, and the average yield was 20mgL(-1). Erwinia herbicola LS005, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis were used to assess the toxicity of ZwA. The antibiotic had strong antibacterial activity against E. herbicola LS005 and a color reaction with ninhydrin.
分类号: Q81`Q5
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