Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P1

文献类型： 外文期刊

第一作者： Pan, Xia

作者： Pan, Xia;Li, Ke;Ma, Rui;Shi, Pengjun;Huang, Huoqing;Yang, Peilong;Meng, Kun;Yao, Bin;Ma, Rui;Yang, Peilong

作者机构：

关键词： Neosartorya fischeri P1;Polygalacturonase;Heterologous expression;Enzyme characterization;Fruit juice processing and clarification

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Polygalacturonase is one of the most important industrial pectinases. To enrich the genetic resources and develop new enzyme candidates, three polygalacturonase genes (Nfpg I-III) of glycosyl hydrolase family 28 were cloned from Neosartorya fischeri P1 and functionally expressed in Pichia pastoris. The purified recombinant proteins exhibited some distinguished properties. In comparison with other counterparts, NfPG I showed the highest specific activity (40, 123 U/mg), NfPG II had the highest temperature optimum (65 degrees C), and the pH optimum of NfPG III was the lowest (3.5). The orders of their thermostability and resistance to chemicals tested were NfPG II > NfPG III > NfPG land NfPG II > NfPG I > NfPG III, respectively. Combinations of these enzymes showed better performance than individuals in the processing and clarification of apple and strawberry juice. These results suggest that N. fischeri polygalacturonases have great application potentials in the food industry for juice production. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： TS2`TS201.2

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