Monitoring tryptophan metabolism after exposure to hexaconazole and the enantioselective metabolism of hexaconazole in rat hepatocytes in vitro

文献类型： 外文期刊

第一作者： Wang, Yao

作者： Wang, Yao;Zhu, Wentao;Wang, Xinru;Zhang, Ping;Yan, Jin;Zhou, Zhiqiang;Qiu, Jing

作者机构：

关键词： Hexaconazole;Enantioselevtive metabolism;Cytotoxicity;Tryptophan metabolism

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：10.588； 五年影响因子：10.129 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present study, the enantioselective metabolism, cytotoxicity of hexaconazole and its influence on tryptophan metabolism in rat hepatocytes in vitro were investigated. Following the exposure of primary rat hepatocytes to rac-hexaconazole, the concentrations of its enantiomers in the media were determined by chiral high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The half-lives (t(1/2)) of (+)-hexaconazole and (-)-hexaconazole were 5.17 hand 19.80 h, respectively, indicating that the metabolic process was enantioselective with (-)-hexaconazole enrichment. Using the MTT method, the EC50 values of rac-hexaconazole, (+)-hexaconazole and (-)-hexaconazole after 12 h of exposure were determined to be 71.62, 62.71 and 67.94 mu M, respectively. Tryptophan metabolism was monitored using metabolomics profiling techniques. Hexaconazole and its enantiomers caused the down-regulation of tryptophan levels and the up-regulation of kynurenine (KYN) levels, suggesting a role for hexaconazole in the activation of the KYN pathway and providing information for the mechanism of its toxicity. (C) 2015 Elsevier B.V. All rights reserved.

分类号： TB1

  • 相关文献

[1]Enantioselective Metabolism and Interference on Tryptophan Metabolism of Myclobutanil in Rat Hepatocytes. Wang, Yao,Zhu, Wentao,Wang, Xinru,Zhang, Ping,Wang, Dezhen,Zhou, Zhiqiang,Qiu, Jing.

[2]Enantioselective Residue Dissipation of Hexaconazole in Cucumber (Cucumis sativus L.), Head Cabbage (Brassica oleracea L. var. caulorapa DC.), and Soils. Wang, Xinquan,Zhang, Hu,Xu, Hao,Wang, Xiangyun,Wu, Changxing,Li, Zhen,Wang, Qiang,Yang, Hongda. 2012

[3]Studies of enantiomeric degradation of the triazole fungicide hexaconazole in tomato, cucumber, and field soil by chiral liquid chromatography-tandem mass spectrometry. Dong, Fengshou,Liu, Xingang,Xu, Jun,Chen, Xiu,Han, Yongtao,Liang, Xuyang,Zheng, Yongquan.

[4]Effects of hexaconazole application on soil microbes community and nitrogen transformations in paddy soils. Xu, Jun,Wu, Xiaohu,Dong, Fengshou,Liu, Xingang,Tian, Chunyan,Zheng, Yongquan.

[5]Dissipation behavior of hexaconazole and kresoxim-methyl residues in ginseng and soil under field conditions. Wang, Yan,Liu, Chang,Gao, Jie,Wang, Chunwei,Cui, Lili,Li, Aijun. 2015

[6]Enhanced Germicidal Efficacy by Co-Delivery of Validamycin and Hexaconazole with Methoxy Poly(ethylene glycol)-Poly(lactide-co-glycolide) Nanoparticles. Zhang, Jiakun,Zhao, Caiyan,Wu, Yan,Zhang, Jiakun,Liu, Yajing,Cao, Lidong,Huang, Qiliang.

[7]Fabrication and stabilization of biocompatible selenium nanoparticles by carboxylic curdlans with various molecular properties. Yan, Jing-Kun,Qiu, Wen-Yi,Wang, Yao-Yao,Wang, Wen-Han,Yang, Yan,Zhang, He-Nan,Wang, Wen-Han,Yang, Yan,Zhang, He-Nan. 2018

[8]Anti-Phytopathogenic and Cytotoxic Activities of Crude Extracts and Secondary Metabolites of Marine-Derived Fungi. Zhao, Dong-Lin,Wang, Dan,Tian, Xue-Ying,Li, Yi-Qiang,Zhang, Cheng-Sheng,Cao, Fei. 2018

[9]Optimization of Catechin Nanoliposomes and Evaluation of Their Antioxidant Activity and Cytotoxicity. Wu, Zhipan,Guan, Rongfa,Liu, Mingqi,Xiao, Chaogeng,Lyu, Fei,Cao, Guozhou,Gao, Jianguo. 2017

[10]Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles. Guan, Rongfa,Kang, Tianshu,Liu, Mingqi,Lu, Fei,Zhang, Zhiguo,Shen, Haitao. 2012

[11]A new drimane sesquiterpenoid glycoside from the seeds of Antiaris toxicaria. Dong, Wen-Hua,Mei, Wen-Li,Zhao, You-Xing,Zeng, Yan-Bo,Wang, Hui,Dai, Hao-Fu,Dong, Wen-Hua,Mei, Wen-Li,Zhao, You-Xing,Zeng, Yan-Bo,Wang, Hui,Dai, Hao-Fu. 2011

[12]Dimeric sesquiterpenoid-4&ITH&IT-chromone derivatives from agarwood of &ITAquilaria crassna&IT and their cytotoxicity. Yang, Yang,Chen, Hui-Qin,Kong, Fan-Dong,Zhou, Li-Man,Li, Wei,Dong, Wen-Hua,Mei, Wen-Li,Dai, Hao-Fu,Yang, Yang,Chen, Zhi-Bao. 2018

[13]Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms. Zheng, Lanhong,Yang, Kangli,Sun, Mi,Zhu, Jiancheng,Lv, Mei,Kang, Daole,Wang, Wei,Xing, Mengxin,Li, Zhao,Liu, Jia. 2016

[14]Cytotoxicity and genotoxicity of 1,4-bisdesoxyquinocetone, 3-methylquinoxaline-2-carboxylic acid (MQCA) in human hepatocytes. Zhang, Keyu,Ban, Manman,Zheng, Haihong,Wang, Xiaoyang,Wang, Mi,Fei, Chenzhong,Xue, Feiqun,Zhao, Zhanzhong. 2012

[15]Ustiloxin G, a New Cyclopeptide Mycotoxin from Rice False Smut Balls. Wang, Xiaohan,Wang, Jian,Lai, Daowan,Wang, Weixuan,Zhou, Ligang,Dai, Jungui,Dai, Jungui,Liu, Yang. 2017

[16]Preparation and immunological effectiveness of a Swine influenza DNA vaccine encapsulated in PLGA microspheres. Li, Guo-Xin,Tong, Guang-Zhi,Zhao, Kai,Jin, Yuan-Yuan,Wei, Hai-Xia,Sun, Qing-Shen,Huang, Ting-Ting,Zhao, Kai,Jin, Yuan-Yuan,Wei, Hai-Xia,Huang, Ting-Ting,Wang, Yun-Feng,Tong, Guang-Zhi. 2010

[17]New Xanthone from the Pericarp of Garcinia mangostana. Fu, Manqin,Xu, Yujuan,Wu, Jijun,Chen, Yulong,Yu, Yuanshan,Xiao, Gengsheng,Qiu, Samuel X.. 2013

[18]A new cytotoxic iridoid from Callicarpa nudiflora. Mei, Wen-Li,Han, Zhuang,Cui, Hai-Bin,Deng, Yuan-Yuan,Dai, Hao-Fu,Zhao, You-Xing. 2010

[19]New Azalomycin F Analogs from Mangrove Streptomyces sp 211726 with Activity against Microbes and Cancer Cells. Yuan, Ganjun,Yuan, Ganjun,Hong, Kui,Lin, Haipeng,Yuan, Ganjun,Hong, Kui,She, Zhigang,Li, Jia. 2013

[20]Cytotoxicity and Apoptosis Induction of Bovine Alpha-lactalbumin-oleic Acid Complex in Human Breast Cancer Cells. Zhong, Saiyi,Chen, Suhua,Zhou, Siyi,Qin, Xiaoming,Wang, Weimin,Liu, Shouchun,Liu, Hai.

作者其他论文 更多>>

微信小程序