A novel major facilitator transporter TrSTR1 is essential for pentose utilization and involved in xylanase induction in Trichoderma reesei

文献类型： 外文期刊

第一作者： Huang, Zhen-Bang

作者： Huang, Zhen-Bang;Chen, Xiu-Zhen;Qin, Li-Na;Wu, Hong-Qing;Dong, Zhi-Yang;Huang, Zhen-Bang;Su, Xiao-Yun

作者机构：

关键词： Trichoderma reesei;Sugar transporter;TrSTR1;Pentose utilization;Xylanase induction

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Trichoderma reesei (teleomorph Hypocrea jecorina) is an industrially important filamentous fungus for glycoside hydrolases production, with its xylanolytic enzymes widely applied in many areas. However, the molecular mechanisms underlying xylanase expression are still insufficiently understood. In particular, the effect of sugar transporter on the induction of xylanase expression is unclear. In this work, we identified a novel major facilitator transporter TrSTR1 that is capable of transporting xylose by using a xylose utilization system in Saccharomyces cerevisiae. In T. reesei, TrSTR1 is essential for the utilization of D-xylose, L-arabinose, and even their downstream metabolites D-xylitol and L-arabitol. TrSTR1 is also involved in the induction of xylanase expression since both the xylanase activity and extracellular protein concentration in the Tu6 Delta str1 strain were decreased, which further confirmed by a qRT-PCR analysis of the transcript levels of the key transcriptional regulators. Our observations provide new insights into connections between pentose utilization and xylanase production in T. reesei (C) 2015 Elsevier Inc. All rights reserved.

分类号： Q5

  • 相关文献

[1]Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei. Wu, Yilan,Sun, Xianhua,Xue, Xianli,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Wu, Yilan,Xie, Xiangming.

[2]Revisiting overexpression of a heterologous beta-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities. Xue, Xianli,Ma, Rui,Luo, Huiying,Su, Xiaoyun,Yao, Bin,Wu, Yilan,Qin, Xing. 2016

[3]Improving cellulase production in submerged fermentation by the expression of a Vitreoscilla hemoglobin in Trichoderma reesei. Lin, Jie,Zhang, Xiamei,Song, Bingran,Xue, Wei,Chen, Xiuzhen,Dong, Zhiyang,Lin, Jie,Zhang, Xiamei,Song, Bingran,Dong, Zhiyang,Su, Xiaoyun. 2017

[4]Efficient Coproduction of Mannanase and Cellulase by the Transformation of a Codon-Optimized Endomannanase Gene from &ITAspergillus niger&IT into &ITTrichoderma reesei &IT. Sun, Xianhua,Xue, Xianli,Li, Mengzhu,Gao, Fei,Hao, Zhenzhen,Huang, Huoqing,Luo, Huiying,Yao, Bin,Su, Xiaoyun,Qin, Lina. 2017

[5]Genome-Wide Characterization and Expression Profiling of Sugar Transporter Family in the Whitefly, Bemisia tabaci (Gennadius) (Hemiptera:Aleyrodidae). Yang, Zezhong,Zheng, Huixin,Yang, Zezhong,Xia, Jixing,Gong, Cheng,Xie, Wen,Guo, Zhaojiang,Zheng, Huixin,Yang, Xin,Wu, Qingjun,Wang, Shaoli,Zhang, Youjun,Xia, Jixing,Pan, Huipeng,Yang, Fengshan. 2017

[6]Genome-wide identification, characterization, and expression analysis of the SWEET gene family in cucumber. Hu Li-ping,Zhang Feng,Song Shu-hui,Tang Xiao-wei,Xu Hui,Liu Guang-min,Wang Ya-gin,He Hong-ju,Hu Li-ping,He Hong-ju. 2017

[7]Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season. Yue, Chuan,Cao, Hong-Li,Wang, Lu,Zhou, Yan-Hua,Huang, Yu-Ting,Hao, Xin-Yuan,Wang, Yu-Chun,Wang, Bo,Yang, Ya-Jun,Wang, Xin-Chao,Yue, Chuan,Cao, Hong-Li,Wang, Lu,Zhou, Yan-Hua,Huang, Yu-Ting,Hao, Xin-Yuan,Wang, Yu-Chun,Wang, Bo,Yang, Ya-Jun,Wang, Xin-Chao,Wang, Lu,Hao, Xin-Yuan,Wang, Yu-Chun,Wang, Bo,Yang, Ya-Jun,Wang, Xin-Chao.

作者其他论文 更多>>

微信小程序