Development of a Rapid Immunochromatographic Lateral Flow Device Capable of Differentiating Phytase Expressed from Recombinant Aspergillus niger phyA2 and Genetically Modified Corn

文献类型： 外文期刊

第一作者： Zhou, Xiaojin

作者： Zhou, Xiaojin;Pu, Ling-Kui;Hui, Elizabeth;Zhang, Jun;Yu, Xiao-Lin;Tu, Zhiguan;Lin, Zhen;Zheng, Jian;Zhang, Jun;Liu, Oi;Liu, Oi;Zhane, Juan

作者机构：

关键词： phytase;glycosylation;monoclonal antibodies;lateral strips;GM corn

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Phytase is a phosphohydrolase considered highly specific for the degradation of phytate to release bound phosphorus for animal consumption and aid in the reduction of environmental nutrient loading. New sources of phytase have been sought that are economically and efficiently productive including the construction of genetically modified (GM) phytase products designed to bypass the costs associated with feed processing. Four monoclonal antibodies (EH10a, FA7, AF9a, and CC1) raised against recombinant Aspergillus niger phyA2 were used to develop a highly specific and sensitive immunochromatographic lateral flow device for rapid detection of transgenic phytase, such as in GM corn. Antibodies sequentially paired and tested along lateral flow strips showed that the EH10aFA7 antibody pair was able to detect the recombinant yeast-phytase at 5 ng/mL, whereas the AF9aCC1 antibody pair to GM phytase corn was able to detect at 2 ng/mL. Concurrent to this development, evidence was revealed which suggests that antibody binding sites may be glycosylated.

分类号： R15`S

  • 相关文献

[1]Growth promoting effect of a transgenic Bacillus mucilaginosus on tobacco planting. Li, Xin,Wu, Zhiqiang,Li, Weidong,Yan, Ruixiang,Li, Li,Li, Jun,Li, Yihang,Li, Minggang.

[2]Immunohistochemical analysis of cell wall hydroxyproline-rich glycoproteins in the roots of resistant and susceptible wax gourd cultivars in response to Fusarium oxysporum f. sp Benincasae infection and fusaric acid treatment. Ma, Li,Xu, Chunxiang,Xie, Dasen,Samaj, Jozef. 2011

[3]Production and evaluation of a panel of monoclonal antibodies against Haemophilus paragallinarum. Zhang, P,Blackall, PJ,Yamaguchi, T,Iritani, Y. 2000

[4]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[5]Generation and characterization of monoclonal antibodies specific for 18 kDa antigen from Taenia solium cysticerci. Zhang, Shaohua,Luo, Xuenong,Guo, Aijiang,Zhu, Xueliang,Cai, Xuepeng.

[6]Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Du, Wenjuan,Wang, Yiping,Huang, Liping,Wei, Yanwu,Chen, Dongjie,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[7]Development of a blocking ELISA for Caprine parainfluenza virus type. Mao, Li,Li, Wenliang,Zhou, Tianci,Yang, Leilei,Hao, Fei,Li, Jizong,Zhang, Wenwen,Jiang, Jieyuan,Mao, Li,Luo, Xuenong. 2017

[8]New Monoclonal Antibodies against a Novel Subtype of Shiga Toxin 1 Produced by Enterobacter cloacae and Their Use in Analysis of Human Serum. Skinner, Craig,Patfield, Stephanie,Khalil, Rowaida,Kong, Qiulian,He, Xiaohua,Khalil, Rowaida,Kong, Qiulian. 2016

[9]Preparation of monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for ornidazole detection. Zhao, Liang,Li, Jiaying,Li, Ye,Wang, Tiangang,Rahman, Ebeydulla,Ji, Baoping,Zhou, Feng,Jin, Xiaolu,Wang, Kai,Xing, Yuan,Xing, Yuan.

[10]Antigenic subtyping and epitopes' competition analysis of porcine circovirus type 2 using monoclonal antibodies. Saha, Dipongkor,Huang, Liping,Bussalleu, Eva,Lefebvre, David J.,Fort, Maria,Nauwynck, Hans J.,Huang, Liping,Van Doorsselaere, Jan.

[11]Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure. Li, Peiwu,Li, Peiwu,Zhang, Qi,Zhang, Wen,Huang, Yanling,Ding, Xiaoxia,Jiang, Jun.

[12]Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Ren, Jian-Le,Zhu, Yuan-Mao,Zhou, Yue-Hui,Lv, Chuang,Yan, Hao,Ma, Lei,Shi, Hong-Fei,Xue, Fei.

[13]Development of a serotype colloidal gold strip using monoclonal antibody for rapid detection type Asia1 foot-and-mouth disease. Lin, Tong,Shao, Jun-jun,Du, Jun-zheng,Cong, Guo-zheng,Gao, Shan-dian,Chang, Huiyun. 2011

[14]Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16. Wang, Wen-Shi,Sun, En-cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai. 2013

[15]Development of Double Antibody Sandwich ELISA for Detection of Duck or Goose Flavivirus. Niu Hui-min,Huang Xin-mei,Han Kai-kai,Liu Yu-zhuo,Zhao Dong-min,Zhang Jing-feng,Liu Fei,Li Tong-tong,Zhou Xiao-bo,Li Yin,Niu Hui-min,Liu Fei,Li Tong-tong,Zhou Xiao-bo,Li Xiang-rui. 2013

[16]Cell Attachment Domains of the Porcine Epidemic Diarrhea Virus Spike Protein Are Key Targets of Neutralizing Antibodies. Li, Chunhua,Li, Chunhua,Li, Wentao,de Esesarte, Eduardo Lucio,Guo, Hongbo,Rottier, Peter J. M.,Bosch, Berend-Jan,van den Elzen, Paul,Aarts, Eduard,van den Born, Erwin.

[17]Characterization of Monoclonal Antibodies against HA Protein of H1N1 Swine Influenza Virus and Protective Efficacy against H1 Viruses in Mice. Liu, Yun,Li, Hongtao,Xue, Yujia,Zhao, Shuang,Li, Chenxi,Qu, Liandong,Zhang, Yun,Liu, Ming. 2017

[18]Ultrastructural changes and the distribution of arabinogalactan proteins during somatic embryogenesis of banana (Musa spp. AAA cv. 'Yueyoukang 1'). Yang, Xiao,Zou, Ru,Chen, Houbin,Xu, Chunxiang,Lin, Guimei,Samaj, Jozef.

[19]Effect of Gelatin Addition on Properties of Pullulan Films. Zhang, Chao,Ma, Yue,Zhao, Xiaoyan,Zhang, Chao,Ma, Yue,Zhao, Xiaoyan,Zhang, Chao,Ma, Yue,Zhao, Xiaoyan,Gao, Dandan. 2013

[20]Characterization of seed proteome in Brachypodium distachyon. Wang, Ke,Han, Xiaofeng,Dong, Kun,Gao, Liyan,Yan, Yueming,Wang, Ke,Han, Xiaofeng,Li, Hongyu,Ye, Xingguo,Ma, Wujun.

作者其他论文 更多>>

微信小程序