Co-prevalance of PMQR and 16S rRNA methylase genes in clinical Escherichia coli isolates with high diversity of CTX-M from diseased farmed pigeons

文献类型： 外文期刊

第一作者： Yang, Ling

作者： Yang, Ling;Yang, Lei;Zhang, Wen-Hui;Ren, Si-Qi;Liu, Ya-Hong;Zeng, Zhen-Ling;Jiang, Hong-Xia;Lu, Dian-Hong;Zhang, Wen-Hui

作者机构：

关键词： E. coli;CTX-M;Multi-drug resistance

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present study, we determined the molecular epidemiology of extended-spectrum (beta-lactamases (ESBLs) in Escherichia coli isolated from diseased farmed pigeons in China. A total of 71 E. coli isolates were collected from three pigeon farms from 2011 to 2012 and screened for the presence of the ESBL genes. The ESBLs producers were further tested for the presence of PMQR-encoding genes as well as the 16S rRNA methylase gene using PCR and DNA sequence analysis. Co-transfer of plasmids encoding for ESBLs, PMQR determinants and/or 16S rRNA methylase gene was performed by conjugation into E. coil. The genetic relatedness and plasmid replicon type were determined. A total of 41 ESBLs producers were identified. Only CTX-M type ESBLs were detected, with the most common CTX-M types being CTX-M-65 (n = 17), CTX-M-27 (n = 11), CTX-M-55 (n = 10). Thirty-eight CTX-M-positive isolates were found to harbor at least one PMQR gene, with aac(6')-Ib-cr (n = 32) and oqxAB (n = 21) being the most prevalent. The rmtB was the only prevalent 16S rRNA methylase gene detected in 24(58.1%) CTX-M-positive isolates. Although most of the CTX-M producers had distinct pulsotypes, clonal transmission in the same farm was observed. blacrx_ NI genes were carried by IncF alone or in combination with IncK plasmids with three different sizes, including 76.8 Kb (n = 20), 194 Kb (n = 5), 104.5 Kb (n = 2). PFGE profiles of CTX-M-positive E. coil isolates indicated potential horizontal spread of these multidrug resistant strains along with those CTX-M encoding genes. Our findings highlight the importance of pigeons as a reservoir of multiple antimicrobial resistance genes. (C) 2015 Elsevier B.V. All rights reserved.

分类号： S8

  • 相关文献

[1]Plasmids of Diverse Inc Groups Disseminate the Fosfomycin Resistance Gene fosA3 among Escherichia coli Isolates from Pigs, Chickens, and Dairy Cows in Northeast China. Wang, Xiu-Mei,Dong, Zhimin,Zhu, Yao,Hua, Xin,Zhang, Yanhe,Liu, Siguo,Zhang, Wan-Jiang,Schwarz, Stefan.

[2]Accurate assessment of antibiotic susceptibility and screening resistant strains of a bacterial population by linear gradient plate. Jin JianLing,Gao PeiJi,Liu YuQing,Li JingRan,Du JiaFa,Hu Ming,Bai Hua,Qi Jing,Gao Chao,Wei TianTian,Su Hong,Li JingRan,Gao Chao,Wei TianTian. 2011

[3]Prevalence and characteristics of rmtB and qepA in Escherichia coli isolated from diseased animals in China. Deng, Yu-Ting,Zeng, Zhen-Ling,Tian, Wei,Yang, Tong,Liu, Jian-Hua,Deng, Yu-Ting. 2013

[4]UV-C inactivation of Escherichia coli and dose uniformity on apricot fruit in a commercial setting. Yan, Ruixiang,Gurtler, Joshua,Sites, Joseph,Fan, Xuetong,Mattheis, James,Yan, Ruixiang. 2014

[5]Accumulation of antibiotics and heavy metals in meat duck deep litter and their role in persistence of antibiotic-resistant Escherichia coli in different flocks on one duck farm. Zhang, X. T.,Ji, X.,Zou, X. T.,Yao, W.,Lin, Y.,Zhao, W.,Shi, Z. D.,Gu, H. R.,Gong, J. S..

[6]In silico analysis and recombinant expression of BamA protein as a universal vaccine against Escherichia coli in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua.

[7]Antibacterial effect of metallic glasses. Lin Bao,Bian XiuFang,Mu Ren,Yang LiFu. 2012

[8]Combination of Sodium Chlorite and Calcium Propionate Reduces Enzymatic Browning and Microbial Population of Fresh-Cut "Granny Smith" Apples. Fan, Xuetong,Guan, Wenqiang. 2010

[9]Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System. Sun, Lichang,He, Tao,Zhang, Lili,Pang, Maoda,Zhou, Yan,Bao, Hongduo,Wang, Ran,Zhang, Qiaoyan.

作者其他论文 更多>>

微信小程序