Selection of reliable reference genes for gene expression studies in Clonostachys rosea 67-1 under sclerotial induction

文献类型： 外文期刊

第一作者： Li, Shi-Dong

作者： Li, Shi-Dong;Sun, Man-Hong

作者机构：

关键词： Clonostachys rosea;Reference gene;Elongation factor;Mycoparasitism;Sclerotinia sclerotiorum;Real-time PCR

期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Reference genes are important to precisely quantify gene expression by real-time PCR. In order to identify stable and reliable expressed genes in mycoparasite Clonostachys rosea indifferent modes of nutrition, seven commonly used housekeeping genes, 18S rRNA, actin, beta-tubulin, elongation factor 1, ubiquitin, ubiquitin-conjugating enzyme and glyceraldehyde-3-phosphate dehydrogenase, from the effective biocontrol isolate C rosea 67-1 were tested for their expression under sclerotial induction and during vegetative growth on PDA medium. Analysis by three software programs showed that differences existed among the candidates. Elongation factor 1 was most stable; the M value in geNorm, SD value in Bestkeeper and stability value in Normfinder analysis were 0.405, 0.450 and 0.442, respectively, indicating that the gene elongation factor 1 could be used to normalize gene expression in C. rosea in both vegetative growth and parasitic process. By using elongation factor 1, the expression of a serine protease gene, sep, in different conditions was assessed, which was consistent with the transcriptomic data. This research provides an effective method to quantitate expression changes of target genes in C rosea, and will assist in further investigation of parasitism-related genes of this fungus. (C) 2015 Elsevier B.V. All rights reserved.

分类号： Q93-3

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