Protective effects against H2O2-induced oxidative damage in lung fibroblast cell by peptide isolated from plasma albumin hydrolysate
文献类型: 外文期刊
第一作者: Wang, Jin-Zhi
作者: Wang, Jin-Zhi;Zhang, Chun-Hui;He, Yu-Tong;Zhang, Hao;Ren, Fa-Zheng;Luo, Jun
作者机构:
关键词: Antioxidative enzyme activities;Peptide fractions;Porcine albumin hydrolysate;Purification;Sequence
期刊名称:EUROPEAN FOOD RESEARCH AND TECHNOLOGY ( 影响因子:2.998; 五年影响因子:3.005 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Antioxidant peptides obtained from alcalase-hydrolyzed porcine plasma albumin (AHA, MW < 3 kD) were purified by consecutive chromatographic methods, and the antioxidative effects of unpurified AHA and sub-fractions from the first step of HPLC (P4, MW < 1.5 kD) and second step of HPLC (P4b, MW < 1.5 kD) were evaluated in cell line. P4 exhibits the highest reducing power (0.89) than its further purified fraction P4b (0.69) and unpurified AHA (0.5). Moreover, a concentration of 100 mg/ml of P4 also demonstrates better protective effect on H2O2-induced oxidative damage and antioxidative enzyme activities (SOD, CAT and GPx) in fibroblast cell than AHA and P4b (P < 0.05). P4 is composed of seven peptides with MW of 500.19, 524.24, 550.23, 568.19, 656.18, 707.44 and 1,022.70, and their amino acids sequences are identified as LIKQ, LQHK, EQKF, PDIPK, KVPQVS, FKDLGE and EHLREKVL, respectively. All the seven peptides contain lysine, indicating that P4 is lysine-rich peptide fraction. We believe these results, with a more pronounced action of P4 than AHA and P4b plus the characterized amino sequences, would lay the foundation of understanding the antioxidative effect of albumin-derived peptides.
分类号: R15
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