Ostrinia furnacalis serpin-3 regulates melanization cascade by inhibiting a prophenoloxidase-activating protease

文献类型: 外文期刊

第一作者: Zhou, Fan

作者: Zhou, Fan;Hong, Fang;An, Chunju;Liu, Yang;Wang, Guirong

作者机构:

关键词: Serpin;Prophenoloxidase activation;Melanization;Immunity

期刊名称:INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY ( 影响因子:4.714; 五年影响因子:4.953 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Serine protease cascade-mediated prophenolxidase activation is a prominent innate immune response in insect defense against the invading pathogens. Serpins regulate this reaction to avoid excessive activation. However, the function of serpins in most insect species, especially in some non-model agriculture insect pests, is largely unknown. We here cloned a full-length cDNA for a serpin, named as serpin-3, from Asian corn borer, Ostrinia furnacalis (Guenee). The open reading frame of serpin-3 encodes 462-amino acid residue protein with a 19-residue signal peptide. It contains a reactive center loop strikingly similar to the proteolytic activation site in prophenoloxidase. Sequence comparison indicates that O. furnacalis serpin-3 is an apparent ortholog of Manduca sexta serpin-3, a defined negative regulator of melanization reaction. Serpin-3 mRNA and protein levels significantly increase after a bacterial or fungal injection. Recombinant serpin-3 efficiently blocks prophenoloxidase activation in larval plasma in a concentration-dependent manner. It forms SDS-stable complexes with serine protease 13 (SP13), and prevents SP13 from cleaving prophenoloxidase. Injection of recombinant serpin-3 into larvae results in decreased fungi-induced melanin synthesis and reduced the expression of attacin, cecropin, gloverin, and peptidoglycan recognition protein-1 genes in the fat body. Altogether, serpin-3 plays important roles in the regulation of prophenoloxidase activation and antimicrobial peptide production in O. furnacalis larvae. (C) 2015 Elsevier Ltd. All rights reserved.

分类号: Q5

  • 相关文献

[1]Serine proteases SP1 and SP13 mediate the melanization response of Asian corn borer, Ostrinia furnacalis, against entomopathogenic fungus Beauveria bassiana. Shen, Dongxu,Hong, Fang,An, Chunju,Liu, Yang,Wang, Guirong.

[2]Characterization and expression profiling of serine protease inhibitors in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Lin, Hailan,Lin, Xijian,Zhu, Jiwei,Yu, Xiao-Qiang,Xia, Xiaofeng,Yang, Guang,You, Minsheng,Lin, Hailan,Lin, Xijian,Zhu, Jiwei,Yu, Xiao-Qiang,Xia, Xiaofeng,Yang, Guang,You, Minsheng,Lin, Hailan,Lin, Xijian,Zhu, Jiwei,Yu, Xiao-Qiang,Xia, Xiaofeng,Yang, Guang,You, Minsheng,Lin, Hailan,Lin, Xijian,Zhu, Jiwei,Xia, Xiaofeng,Yang, Guang,You, Minsheng,Lin, Hailan,Lin, Xijian,Zhu, Jiwei,Xia, Xiaofeng,Yang, Guang,You, Minsheng,Yu, Xiao-Qiang,Yao, Fengluan. 2017

[3]Isolation and characterization of two novel serpins from the tick Rhipicephalus haemaphysaloides. Yu, Yingfang,Cao, Jie,Zhou, Yongzhi,Zhang, Honshuang,Zhou, Jinlin. 2013

[4]Studying the Mechanism of Plasmopara viticola RxLR Effectors on Suppressing Plant Immunity. Li, Xinlong,Wu, Jiao,Zhang, Yali,Lu, Jiang,Yin, Ling. 2016

[5]Transgenic Eimeria magna Perard, 1925 Displays similar Parasitological Properties to the Wild-type strain and induces an exogenous Protein-specific immune response in rabbits (Oryctolagus cuniculus L.). Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Shi, Tuanyuan,Duszynski, Donald W.. 2017

[6]Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels. Zhou, Chuanpeng,Ge, Xianping,Liu, Bo,Xie, Jun,Chen, Ruli,Zhou, Chuanpeng. 2014

[7]Effect of High Dietary Carbohydrate on Growth, Serum Physiological Response, and Hepatic Heat Shock Protein 70 Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures. Zhou, Chuanpeng,Liu, Bo,Xie, Jun,Ge, Xianping,Liu, Bo,Xie, Jun,Ge, Xianping. 2013

[8]Transcriptome analysis of the spleen of the darkbarbel catfish Pelteobagrus vachellii in response to Aeromonas hydrophila infection. Wen, Zhengyong,Yuan, Dengyue,Shao, Ting,Wang, Jun,Li, Huatao,Gong, Quan. 2017

[9]Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus). Yang, Chang-Geng,Sun, Bing,Liu, Shan-Shan,Chen, Song-Lin,Yang, Chang-Geng,Wang, Xian-Li,Zhang, Bo. 2013

[10]Identification and screening of effective protective antigens for channel catfish against Streptococcus iniae. Wang, Yajun,Wang, Erlong,He, Yang,Wang, Kaiyu,Yang, Qian,Wang, Jun,Geng, Yi,Ouyang, Ping,Lai, Weimin,Wang, Yajun,Wang, Kaiyu,Geng, Yi,Chen, Defang,Huang, Xiaoli. 2017

[11]Molecular characterization and expression analysis of a novel r-spondin member (rspo2l) in Chinese tongue sole (Cynoglossus semilaevis). Wei, Min,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Li, Hai-long. 2018

[12]Multiplex immune-related genes expression analysis response to bacterial challenge in mud crab, Scylla paramamosain. Zhang, Fengying,Jiang, Keji,Sun, Manman,Zhang, Dan,Ma, Lingbo. 2013

[13]The warm temperature acclimation protein Wap65 as an immune response gene: Its duplicates are differentially regulated by temperature and bacterial infections. Sha, Zhenxia,Yu, Peng,Takano, Tomokazu,Liu, Hong,Terhune, Jeffrey,Liu, Zhanjiang,Sha, Zhenxia,Liu, Hong. 2008

[14]Protochordate amphioxus is an emerging model organism for comparative immunology. Zhang, Shicui,Liang, Yujun,Ji, Guangdong,Zhuang, Zhimeng. 2009

[15]Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in Turbot (Scophthalmus maximus). Yang, Chang-Geng,Liu, Shan-Shan,Sun, Bing,Wang, Na,Chen, Song-Lin,Yang, Chang-Geng,Sun, Bing,Wang, Xian-Li. 2013

[16]Influence of Ligustrum lucidum and Schisandra chinensis fruits on antioxidative metabolism and immunological parameters of layer chicks. Ma, Deying,Liu, Yuqin,Liu, Shengwang,Li, Qundao,Shan, Anshan. 2007

[17]Differential physiological effects of neonicotinoid insecticides on honey bees: A comparison between Apis mellifera and Apis cerana. Li, Zhiguo,Li, Meng,He, Jingfang,Zhao, Xiaomeng,Huang, Wei-Fone,Nie, Hongyi,Zhao, Yazhou,Su, Songkun,Chaimanee, Veeranan,Zhao, Yazhou. 2017

[18]Effects of Antibacterial Peptide Extracted from Bacillus subtilis fmbJ on the Growth, Physiological Response and Disease Resistance of Megalobrama amblycephala. He, Yijin,Liu, Bo,Xie, Jun,Ge, Xianping,Xu, Pao,Lu, Ying,Lu, Fengxia,Lu, Zhaoxin. 2014

[19]Effects of different forms of yeast Saccharomyces cerevisiae on growth performance, intestinal development, and systemic immunity in early-weaned piglets. Jiang, Zongyong,Wei, Shaoyong,Wang, Zhilin,Hu, Shenglan,Zheng, Chuntian,Hu, Youjun,Wang, Li,Ma, Xianyong,Yang, Xuefen,Jiang, Zongyong,Zhu, Cui,Chen, Zhuang. 2015

[20]The effect of essential oils on performance, immunity and gut microbial population in weaner pigs. Ru, Y. J.,Peron, A.,Li, S. Y.,Liu, M.,Xu, B.,Shi, X. G.. 2012

作者其他论文 更多>>