Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides)

文献类型： 外文期刊

第一作者： Shi, Yan

作者： Shi, Yan;Hong, Xiaoyou;Chen, Kunci;Zhu, Xinping;Zhao, Zhe

作者机构：

关键词： VDAC1;Epinephelus coioides;Tissue Expression;Subcellular Localization;Overexpression

期刊名称：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY （ 影响因子：3.642； 五年影响因子：3.222 ）

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收录情况： SCI

摘要： The voltage-dependent anion channel (VDAC) is a highly conserved integral protein of mitochondria in different eukaryotic species. It forms a selective channel in the mitochondrial outer membrane that serves as the controlled pathway for small metabolites and ions. In this study, a VDAC gene, EcVDAC1, was isolated from orange-spotted grouper (Epinephelus coioides). The EcVDAC1 exhibits ubiquitous expression in various tissues of orange-spotted grouper and is upregulated in liver, gill, and spleen after stimulation with lipopolysaccharides (LPS). Subcellular localization analysis shows that the EcVDAC1 protein colocalized with the mitochondria. A caspase-3 assay demonstrates that overexpression of the EcVDAC1 induced apoptotic cell death in fathead minnow cells. The data presented in this study provide new information regarding the relationship between LPS and the EcVDAC1 gene, suggesting that the fish VDAC1 gene may play an important role in antibacterial immune response. (C) 2014 Wiley Periodicals, Inc.

分类号： Q5

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