Detection of genetic variations in Orf virus isolates epidemic in Xinjiang China

文献类型: 外文期刊

第一作者: Yang, Haibo

作者: Yang, Haibo;Meng, Qingling;Qiao, Jun;Peng, Yelong;Xie, Kun;Liu, Yucheng;Zhao, Hailong;Chen, Chuangfu;Cai, Xuepeng

作者机构:

关键词: Genetic variability;Parapoxvirus;Orf virus;Zoonosis;Contagious ecthyma;Domestic ruminants;Sheep and goats

期刊名称:JOURNAL OF BASIC MICROBIOLOGY ( 影响因子:2.281; 五年影响因子:2.516 )

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年卷期:

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收录情况: SCI

摘要: Orf is an exanthemous viral disease seriously threatening the goat and sheep industry and widely epidemic in the goat and sheep populations in Xinjiang, China. In order to investigate the genetic variability of the orf virus (ORFV), three virus isolates (SHZ1, SHZ2, and SHZ3) were isolated by PCR and Vero cell culture using the clinical samples from the lips of the lambs suspected of ORFV infection. The isolates were further verified by electron microscopy and animal infection experiments. The protective antigen genes B2L, F1L, and virulence genes VIR, GIF, and VEGF in the isolates were cloned, sequenced and analyzed for genetic evolution. The results showed that B2L and F1L were relatively conservative with homology 86.7-97.9%, while VIR, GIF, particularly VEGF were considerably variable with homology 71.5-97.9% at amino acid sequence level, respectively. Phylogenetic tree analysis based on B2L and VIR showed that the isolates SHZ1 and SHZ2 were closely related with the Taiwan isolates. This is the first report to confirm that there have been genetic variations in the Xinjiang ORFV isolates. The findings provide molecular evidence about the genetic variability of the major antigenic and virulence genes in the virus isolates epidemic in Xinjiang.

分类号: R37

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