Molecular characterization of insulin-like androgenic gland hormone-binding protein gene from the oriental river prawn Macrobrachium nipponense and investigation of its transcriptional relationship with the insulin-like androgenic gland hormone gene

文献类型： 外文期刊

第一作者： Li, Fajun

作者： Li, Fajun;Bai, Hongkun;Fu, Hongtuo;Jiang, Fengwei;Li, Fajun;Xiong, Yiwei;Jiang, Sufei;Jin, Shubo;Sun, Shengming;Qiao, Hui;Zhang, Wenyi

作者机构：

关键词： Macrobrachium nipponense;Insulin-like androgenic gland hormone-binding protein gene;Insulin-like androgenic gland hormone gene;RNA interference

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

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收录情况： SCI

摘要： Insulin-like androgenic gland hormone-binding protein (IAGBP) has been investigated in crustaceans in vitro. However, the relationship between IAGBP and its putative binding protein partner insulin-like androgenic gland hormone (IAG) has not been studied at the transcriptional level in vivo. In the current study, we cloned the full-length cDNA of IAGBP from the oriental river prawn Macrobrachium nipponense (Mn-IAGBP) and investigated the transcriptional patterns of Mn-IAGBP and the M. nipponense TAG gene (Mn-IAG) at different developmental stages and in different tissues. Mn-IAGBP mRNA was detected in all examined tissues from adult male prawns, with the highest transcriptional levels in the testis. Mn-IAG mRNA was detected in the androgenic gland and hepatopancreas. The genomic sequences of Mn-IAGBP and Mn-IAG were isolated by genome walking and two gene copies were found in both Mn-IAGBP and Mn-IAG. The relationship between Mn-IAGBP and Mn-IAG at the transcriptional level was studied by RNA interference. Injection of Mn-IAGBP double-stranded RNA (dsRNA) significantly reduced the transcription of Mn-IAG, while injection of Mn-IAG dsRNA significantly reduced the transcription of Mn-IAGBP in testis, muscle, androgenic gland, and hepatopancreas. These results demonstrate the involvement of the IAGBP gene in JAG signaling in M. nipponense. (C) 2014 Elsevier Inc. All rights reserved.

分类号： R58

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