Crucial Genes and Pathways in Chicken Germ Stem Cell Differentiation

文献类型： 外文期刊

第一作者： Zhang, Zhentao

作者： Zhang, Zhentao;Elsayed, Ahmed Kamel;Shi, Qingqing;Zhang, Yani;Zuo, Qisheng;Li, Dong;Lian, Chao;Tang, Beibei;Xiao, Tianrong;Xu, Qi;Chang, Guobin;Chen, Guohong;Zhang, Lei;Wang, Yingjie;Jin, Kai;Wang, Yilin;Cui, Hengmi;Li, Bichun;Wang, Kehua;Song, Jiuzhou;Elsayed, Ahmed Kamel

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期刊名称：JOURNAL OF BIOLOGICAL CHEMISTRY （ 影响因子：5.157； 五年影响因子：5.041 ）

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收录情况： SCI

摘要： Male germ cell differentiation is a subtle and complex regulatory process. Currently, its regulatory mechanism is still not fully understood. In our experiment, we performed the first comprehensive genome and transcriptome-wide analyses of the crucial genes and signaling pathways in three kinds of crucial cells (embryonic stem cells, primordial germ cell, and spermatogonial stem cells) that are associated with the male germ cell differentiation. We identified thousands of differentially expressed genes in this process, and from these we chose 173 candidate genes, of which 98 genes were involved in cell differentiation, 19 were involved in the metabolic process, and 56 were involved in the differentiation and metabolic processes, like GAL9, AMH, PLK1, and PSMD7 and so on. In addition, we found that 18 key signaling pathways were involved mainly in cell proliferation, differentiation, and signal transduction processes like TGF-beta, Notch, and Jak-STAT. Further exploration found that the candidate gene expression patterns were the same between in vitro induction experiments and transcriptome results. Our results yield clues to the mechanistic basis of male germ cell differentiation and provide an important reference for further studies.

分类号： Q5

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