Evaluation of a novel ultra-sensitive nanoparticle probe-based assay for ricin detection

文献类型： 外文期刊

第一作者： Yin, Hui-qiong

作者： Yin, Hui-qiong;Jia, Min-xian;Liu, Jun;Wang, Rui;Lv, Mao-min;Ma, Yu-yuan;Zhao, Xiong;Zhang, Jin-gang;Shi, Li-jun

作者机构：

关键词： Gold nanoparticle probe-based assay;high-sensitivity detection;real-time PCR;ricin

期刊名称：JOURNAL OF IMMUNOTOXICOLOGY （ 影响因子：3.0； 五年影响因子：2.597 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A gold nanoparticle (GNP) probe-based assay (GNPA) modified from the bio-barcode assay (BCA) was developed for ultrasensitive detection of ricin, a potential biothreat agent. In the GNPA, a chain of ricin was captured by a GNP probe coated with polyclonal antibodies and single-stranded signal DNA. A magnetic microparticle (MMP) probe coated with ricin A chain monoclonal antibody was then added to form an immuno-complex. After being magnetically separated, the immuno-complex containing the single-stranded signal DNA was characterized by PCR and real-time PCR. A detection limit of 10(-2) fg/ml was determined for the ricin A chain; this is eight orders of magnitude more sensitive than that achieved with an ELISA and two orders more sensitive than that obtained with the BCA. The coefficients of variation (CV) of the intra-and inter-assay values ranged from 3.82-6.46%. The results here show that this novel assay is an ultrasensitive method for detection of ricin proteins and may be suitable for the ultrasensitive detection of other proteins.

分类号： R39

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