Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes

文献类型： 外文期刊

第一作者： Ye, Chao

作者： Ye, Chao;Tian, Zhi-Jun;Zhao, Kuan;Guo, Jin-Chao;Jiang, Cheng-Gang;Wang, Shu-Jie;Chang, Xiao-Bo;Peng, Jin-Mei;Tang, Yan-Dong;Sun, Ming-Xia;Cai, Xue-Hui;An, Tong-Qing;Zhang, Qing-Zhan;Zheng, Hao;Liu, Fei;Tong, Wu;Jiang, Yi-Feng;Zhou, Yan-Jun;Tong, Guang-Zhi;Shi, Mang;Shi, Mang

作者机构：

关键词： PRV;Divergence;RFLP;Microsatellite;Genotype

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Recently pseudorabies outbreaks have occurred in many vaccinated farms in China. To identify genetic characteristics of pseudorabies virus (PRV) strains, we obtained the genomic sequences of PRV strains HeN1 and JS, which were compared to 4 PRV genomes and 729 partial gene sequences. PRV strains isolated in China showed marked sequence divergence compared to European and American strains. Phylogenetic analysis revealed that for the first time PRV can be divided into 2 distinct clusters, with Chinese strains being genotype II and PRVs isolated from other countries being genotype I. Restriction fragment length polymorphism analysis confirmed differences between HeN1 and Bartha strains, as did the presence of unique insertion/deletion polymorphisms and microsatellites. This divergence between the two genotypes may have been generated from long-term, independent evolution, which could also explain the low efficacy of the Bartha vaccine in protecting pigs infected with genotype II PRV. (C) 2015 Elsevier Inc. All rights reserved.

分类号： R37

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