Rhoptry-associated protein (rap-1) genes in the sheep pathogen Babesia sp Xinjiang: Multiple transcribed copies differing by 3 ' end repeated sequences

文献类型： 外文期刊

第一作者： Niu, Qingli

作者： Niu, Qingli;Marchand, Jordan;Bonsergent, Claire;Malandrin, Laurence;Niu, Qingli;Marchand, Jordan;Bonsergent, Claire;Malandrin, Laurence;Niu, Qingli;Yang, Congshan;Guan, Guiquan;Yin, Hong

作者机构：

关键词： Babesia;Sheep;Rhoptry-associated protein 1;Multigene family;Amino acid repeats

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Sheep babesiosis occurs mainly in tropical and subtropical areas. The sheep parasite Babesia sp. Xinjiang is widespread in China, and our goal is to characterize rap-1 (rhoptry-associated protein 1) gene diversity and expression as a first step of a long term goal aiming at developing a recombinant subunit vaccine. Seven different rap-1a genes were amplified in Babesia sp. Xinjiang, using degenerate primers designed from conserved motifs. Rap-1b and rap-1c gene types could not be identified. In all seven rap-la genes, the 5' regions exhibited identical sequences over 936 nt, and the 3' regions differed at 28 positions over 147 nt, defining two types of genes designated alpha and beta. The remaining 3' part varied from 72 to 360 nt in length, depending on the gene. This region consists of a succession of two to ten 36 nt repeats, which explains the size differences. Even if the nucleotide sequences varied, 6 repeats encoded the same stretch of amino acids. Transcription of at least four a and two beta genes was demonstrated by standard RT-PCR. (C) 2015 Elsevier B.V. All rights reserved.

分类号： S85

  • 相关文献

[1]Course of infection by Babesia sp BQ1 (Lintan) and B-divergens in sheep depends on the production of IFN gamma and IL10. Moreau, E.,Guan, G.,Yin, H.,Luo, J.. 2010

[2]Discrimination between ovine Babesia and Theileria species in China based on the ribosomal protein S8 (RPS8) gene. Tian, Zhancheng,Liu, Guangyuan,Yin, Hong,Luo, Jianxun,Guan, Guiquan,Luo, Jin,Xie, Junren,Zheng, Jinfeng,Yuan, Xiaosong,Wang, Fangfang,Shen, Hui,Tian, Meiyuan. 2013

[3]Molecular investigation of piroplasma infection in white yaks (Bos grunniens) in Gansu province, China. Li, Sitong,Liu, Junlong,Liu, Aihong,Li, Youquan,Yin, Hong,Luo, Jianxun,Guan, Guiquan,Wang, Shuqing,Wang, Shufang,Yin, Hong. 2017

[4]Phylogenetic analysis based on 28S rRNA of Babesia spp. in ruminants in China. Gou, Huitian,Guan, Guiquan,Ma, Miling,Liu, Aihong,Liu, Zhijie,Ren, Qiaoyun,Li, Youquan,Yang, Jifei,Chen, Ze,Yin, Hong,Luo, Jianxun. 2013

[5]A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B-venatorum from White Yaks in Tianzhu, China. Liu, Junlong,Guan, Guiquan,Li, Youquan,Liu, Aihong,Luo, Jianxun,Yin, Hong,Liu, Junlong,Guan, Guiquan,Li, Youquan,Liu, Aihong,Luo, Jianxun,Yin, Hong. 2017

[6]Babesia sp. BQ1 (Lintan): Molecular evidence of experimental transmission to sheep by Haemaphysalis qinghaiensis and Haemaphysalis longicornis. Guan, Guiquan,Moreau, Emmanuelle,Chauvin, Alain,Guan, Guiquan,Liu, Junlong,Hao, Xuefen,Ma, Miling,Luo, Jianxun,Yin, Hong.

[7]Molecular evidence for piroplasms in wild Reeves' muntjac (Muntiacus reevesi) in China. Yang, Ji-fei,Li, You-quan,Liu, Zhi-jie,Liu, Jun-long,Guan, Gui-quan,Chen, Ze,Luo, Jian-xun,Wang, Xiao-long,Yin, Hong,Wang, Xiao-long.

[8]Molecular detection and identification of piroplasms in sika deer (Cervus nippon) from Jilin Province, China. Liu, Junlong,Yang, Jifei,Guan, Guiquan,Liu, Aihong,Wang, Bingjie,Luo, Jianxun,Yin, Hong,Liu, Junlong,Yang, Jifei,Guan, Guiquan,Liu, Aihong,Wang, Bingjie,Luo, Jianxun,Yin, Hong. 2016

[9]Cytochrome c oxidase subunit III (COX3) gene, an informative marker for phylogenetic analysis and differentiation of Babesia species in China. Tian, Zhancheng,Liu, Guangyuan,Yin, Hong,Luo, Jianxun,Guan, Guiquan,Xie, Junren,Luo, Jin,Zheng, Jinfeng,Tian, Meiyuan,Yuan, Xiaosong,Wang, Fangfang,Chen, Ronggui,Wang, Haijun.

[10]Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China. Niu, Qingli,Liu, Zhijie,Yu, Peifa,Yang, Jifei,Abdallah, Mirza Omar,Guan, Guiquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2015

[11]Polymorphism of exon 2 of DIO2 gene and its association with seasonal reproduction in sheep. He, JianNing,Huang, DongWei,Di, Ran,Wang, JinXin,Chu, MingXing,Liu, QiuYue,Hu, WenPing,Wang, XiangYu,Pan, ZhangYuan,He, JianNing. 2016

[12]Protecting Local Breeds of Livestock in NW China. Lang Xia,Wang Cailian,Squires, Victor. 2010

[13]Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe. Hu Hong-lian,Lu De-xun,Gao Min,Liu Yong-zhi,Liu Yong-zhi,Li Ya-kui. 2014

[14]Cloning and characterization of ovine immunoglobulin G Fc receptor II (Fc gamma RII). Liu, Yunchao,Qiao, Songlin,Zhang, Gaiping,Xi, Jun,You, Leiming,Tian, Xiaohui,Li, Qiaomu,Zhang, Lina,Guo, Junqing,Liu, Yunchao,Wang, Aiping. 2010

[15]Progress in biotechnology of small ruminants in China. Zhu, YD,Guo, Y,Ni, HM. 1999

[16]Anaplasma phagocytophilum in sheep and goats in central and southeastern China. Yang, Jifei,Liu, Zhijie,Niu, Qingli,Liu, Junlong,Han, Rong,Guan, Guiquan,Li, Youquan,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2016

[17]Seroprevalence, Isolation, Genotyping, and Pathogenicity of Toxoplasma gondii Strains from Sheep in China. Yang, YuRong,Feng, YongJie,Lu, YaoYao,Liang, HongDe,Zhang, LongXian,Yao, QiuXia,Zhu, XingQuan,Wang, YingHua. 2017

[18]Molecular detection and genetic characterization of Babesia, Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey. Zhou, Mo,Cao, Shinuo,Jirapattharasate, Charoonluk,Moumouni, Paul Franck Adjou,Liu, Mingming,Wang, Guanbo,Iguchi, Aiko,Vudriko, Patrick,Suzuki, Hiroshi,Xuan, Xuenan,Zhou, Mo,Cao, Shinuo,Sevinc, Ferda,Sevinc, Mutlu,Ceylan, Onur,Ekici, Sepil. 2017

[19]Polymorphisms of coding region of BMPR-IB gene and their relationship with litter size in sheep. Chu, Mingxing,Fang, Li,Di, Ran,Cao, Guiling,Feng, Tao,Tang, Qianqian,Ma, Yuehui,Li, Kui,Jia, Lihua,Chen, Hongquan,Zhang, Yingjie,Jin, Mei. 2011

[20]Ovine prion protein genotype frequencies in northwestern China. Zhao, C. -L.,Wu, R.,Liu, L.,Zhang, X. -L.,Wang, C.,Wang, F.,Diao, X. -L.,Guan, H. -W.,Wang, X.,Zhou, L.,Wu, R.,Li, F. -D.. 2012

作者其他论文 更多>>
  • Epidemiology, molecular diversity and zoonotic implications of Echinococcus granulosus sensu stricto (G1) in cattle across Nigeria: Evidence of broader transmission dynamics

    作者：Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Morenikeji, Olajumoke Abimbola;Odaibo, Georgina Nijideka;Onoja, Bernard Anyebe;Akanbi, Ibikunle Mark;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong

    关键词：Echinococcus granulosus sensu lato; Cystic echinococcosis; Nigeria; Genetic diversity; Molecular epidemiology; One health

  • A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China

    作者：Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Hou, Xue-Yi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Guo, Min-Kuan;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Shumuye, Nigus Abebe;Gao, Sheng-Zhi;Hou, Xue-Yi;Zhonggecao, Tao;Zhaxilamao, Bao-Quan;Gazangzhuoma, Hong;Doukao, Hong-Bin;Zhang, Fu-Heng;Guo, Min-Kuan;Li, Wen-Dong;Yang, Tao;Fu, Bao-Quan;Yin, Hong;Jia, Wan-Zhong

    关键词：Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity

  • Expanding the molecular understanding of Theileria infections in cattle and yaks from Gansu and Qinghai, China

    作者：Chai, Yijun;Che, Jin;Zhao, Shuaiyang;Wang, Jinming;Guan, Guiquan;Yin, Hong;Che, Jin;Yin, Hong

    关键词：Molecular prevalence; Theileriosis; Piroplasm; Bovine

  • Lacticaseibacillus rhamnosus G7 alleviates DSS-induced ulcerative colitis by regulating the intestinal microbiota

    作者：Lao, Jianlong;Chen, Man;Yan, Shuping;Gong, Han;Wen, Zhaohai;Yong, Yanhong;Jia, Dan;Lv, Shuting;Zou, Wenli;Li, Junmei;Tan, Huiming;Liu, Zengyuan;Guo, Fucheng;Ju, Xianghong;Li, Youquan;Lao, Jianlong;Ju, Xianghong;Li, Youquan;Jia, Dan;Yin, Hong;Kong, Xiangying

    关键词：Probiotics; Inflammatory; Lacticaseibacillus rhamnosus G7; Gut microbiota

  • First determination of diagnostic doses of the pesticide-sensitive strain of Haemaphysalis longicornis in China

    作者：Diao, Peiwen;Luo, Jin;Ren, Qiaoyun;Wang, Jinming;Cao, Runlai;Guo, Junhui;Guan, Guiquan;Luo, Jianxun;Yin, Hong;Liu, Guangliang;Liu, Guangyuan;Ren, Qiaoyun;Zhang, Yan;Luo, Jianxun;Yin, Hong

    关键词：ticks; pesticide-sensitive strain; Haemaphysalis longicornis; discriminating concentration

  • The African swine fever virus gene MGF_360-4L inhibits interferon signaling by recruiting mitochondrial selective autophagy receptor SQSTM1 degrading MDA5 antagonizing innate immune responses

    作者：Sun, Hualin;Yang, Jifei;Zhang, Zhonghui;Wu, Mengli;Tian, Zhancheng;Liu, Ying;Zhang, Xiaoqiang;Zhong, Jianhao;Yang, Songlin;Chen, Yikang;Luo, Jianxun;Guan, Guiquan;Yin, Hong;Niu, Qingli;Sun, Hualin;Yang, Jifei;Zhang, Zhonghui;Wu, Mengli;Tian, Zhancheng;Liu, Ying;Zhang, Xiaoqiang;Zhong, Jianhao;Yang, Songlin;Chen, Yikang;Luo, Jianxun;Guan, Guiquan;Yin, Hong;Niu, Qingli;Yin, Hong

    关键词：ASFV; MGF_360-4L; SQSTM1; MDA5; mitochondrial autophagy

  • Comparative microRNAs profile of Schistosoma japonicum male worms derived from single-sex and bisexual infections: Implications of the multifunctional role of microRNA

    作者：Zhong, Haoran;Zhu, Danlin;Dong, Bowen;Wu, Luobin;Lu, Ke;Fu, Zhiqiang;Liu, Jinming;Jin, Yamei;Wu, Luobin;Guan, Guiquan;Guan, Guiquan

    关键词：Schistosoma japonicum; MiRNA; Single-sex male worms; Mated male worms

微信小程序