Rhoptry-associated protein (rap-1) genes in the sheep pathogen Babesia sp Xinjiang: Multiple transcribed copies differing by 3 ' end repeated sequences

文献类型： 外文期刊

第一作者： Niu, Qingli

作者： Niu, Qingli;Marchand, Jordan;Bonsergent, Claire;Malandrin, Laurence;Niu, Qingli;Marchand, Jordan;Bonsergent, Claire;Malandrin, Laurence;Niu, Qingli;Yang, Congshan;Guan, Guiquan;Yin, Hong

作者机构：

关键词： Babesia;Sheep;Rhoptry-associated protein 1;Multigene family;Amino acid repeats

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Sheep babesiosis occurs mainly in tropical and subtropical areas. The sheep parasite Babesia sp. Xinjiang is widespread in China, and our goal is to characterize rap-1 (rhoptry-associated protein 1) gene diversity and expression as a first step of a long term goal aiming at developing a recombinant subunit vaccine. Seven different rap-1a genes were amplified in Babesia sp. Xinjiang, using degenerate primers designed from conserved motifs. Rap-1b and rap-1c gene types could not be identified. In all seven rap-la genes, the 5' regions exhibited identical sequences over 936 nt, and the 3' regions differed at 28 positions over 147 nt, defining two types of genes designated alpha and beta. The remaining 3' part varied from 72 to 360 nt in length, depending on the gene. This region consists of a succession of two to ten 36 nt repeats, which explains the size differences. Even if the nucleotide sequences varied, 6 repeats encoded the same stretch of amino acids. Transcription of at least four a and two beta genes was demonstrated by standard RT-PCR. (C) 2015 Elsevier B.V. All rights reserved.

分类号： S85

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