A Fungal Alpha-Galactosidase from Pseudobalsamia microspora Capable of Degrading Raffinose Family Oligosaccharides

文献类型: 外文期刊

第一作者: Yang, Dongxue

作者: Yang, Dongxue;Du, Fang;Wang, Hexiang;Yang, Dongxue;Du, Fang;Wang, Hexiang;Tian, Guoting;Zhao, Yongchang;Zhao, Liyan;Tzi Bun Ng

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关键词: Pseudobalsamia microspora;Purification and characterization;Alpha-galactosidase;Chemical modification;Degradation

期刊名称:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ( 影响因子:2.926; 五年影响因子:2.685 )

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收录情况: SCI

摘要: An alpha-galactosidase was purified from Pseudobalsamia microspora (PMG) to 1224.1-fold with a specific activity of 11,274.5 units/mg by ion-exchange chromatography and gel filtration. PMG is a monomeric protein with a molecular mass of 62 kDa as determined by SDS-PAGE and by gel filtration. Chemical modification using N-bromosuccinimide (NBS) resulted in a complete abrogation of the activity of PMG, suggesting that Trp is an amino acid essential to its activity. The activity was strongly inhibited by Hg2+, Cd2+, Cu2+, and Fe3+ ions. Three inner peptide sequences for PMG were obtained by liquid chromatography-tandem mass spectrometry (LC-MS-MS) analysis. When 4-nitrophenyl alpha-d-glucopyranoside (pNPGal) was used as substrate, the optimum pH and temperature of PMG were 5.0 and 55 A degrees C, respectively. The Michaelis constant (K (m)) value of the alpha-galactosidase on pNPGal was 0.29 mM, and the maximal velocity (V (max)) was 0.97 mu mol ml(-1) min(-1). Investigation by thin-layer chromatography (TLC) demonstrated its ability to hydrolyze raffinose and stachyose. Hence, it can be exploited in degradation of non-digestible oligosaccharides from food and feed industries.

分类号: Q5

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