Selection of appropriate reference genes in eggplant for quantitative gene expression studies under different experimental conditions

文献类型： 外文期刊

第一作者： Zhou, Xiaohui

作者： Zhou, Xiaohui;Liu, Jun;Zhuang, Yong

作者机构：

关键词： Quantitative real-time PCR;Reference gene;Gene expression;Eggplant (Solanum melongena L.)

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Quantitative real-time polymerase chain reaction (RT-qPCR) has become one of the most widely used methods for gene expression analysis, and the evaluation of appropriate reference genes should be the first step to consider in experiments based on RT-qPCR. In this work, nine candidate reference genes, including 18sRNA, CYP, UBQ GAPDH, TUB, TUA, EF1, ACTIN and RPOB, were investigated for their expression stability in five different eggplant sample pools using three algorithms: geNorm, NormFinder and RefFinder. The samples were collected under different experimental conditions, including those from different plant tissues, hormone treatments, biotic and abiotic stresses. Our results demonstrated that the expression stability varied from different experimental conditions between reference genes. In general, GAPDH and 18sRNA had a good performance under most experimental conditions, whereas TUB and RPOB exhibited poor stability. This study should provide guidelines for the selection of reference genes for gene expression studies in eggplant. (C) 2014 Elsevier B.V. All rights reserved.

分类号： S6

  • 相关文献

[1]Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR. Ye, Xing,Zhang, Lili,Tian, Yuanyuan,Lao, Haihua,Bai, Junjie,Yu, Lingyun,Dong, Haiyan,Dong, Haiyan.

[2]Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole (Cynoglossus semilaevis). Ma Qian,Zhuang Zhimeng,Feng Wenrong,Liu Shufang,Tang Qisheng. 2015

[3]Validation of Reference Genes for Quantitative Real-Time PCR during Bicolor Tepal Development in Asiatic Hybrid Lilies (Lilium spp.). Xu, Leifeng,Xu, Hua,Cao, Yuwei,Yang, Panpan,Feng, Yayan,Tang, Yuchao,Yuan, Suxia,Ming, Jun,Yang, Panpan. 2017

[4]Selection of Reference Genes for Expression Analysis of Kumamoto and Portuguese Oysters and Their Hybrid. Yan Lulu,Wang Zhaoping,Yu Ruihai,Yan Lulu,Su Jiaqi,Yan Xiwu. 2017

[5]Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz. Yi, Shanjun,Qian, Yongqiang,Han, Lei,Sun, Zhenyuan,Liu, Junxiang,Ju, Guansheng,Fan, Chengming.

[6]Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time PCR. Hu, Meizhen,Wang, Wenquan,Hu, Meizhen,Xia, Zhiqiang,Zhou, Xincheng,Wang, Wenquan,Hu, Meizhen,Wang, Wenquan,Hu, Wenbin. 2016

[7]Identification and testing of reference genes for Sesame gene expression analysis by quantitative real-time PCR. Wei, Libin,Miao, Hongmei,Zhao, Ruihong,Han, Xiuhua,Zhang, Tide,Zhang, Haiyang.

[8]Reference genes selection for transcript normalization in kenaf (Hibiscus cannabinus L.) under salinity and drought stress. Niu, Xiaoping,Qi, Jianmin,Chen, Meixia,Tao, Aifen,Fang, Pingping,Xu, Jiantang,Onyedinma, Sandra A.,Chen, Meixia,Zhang, Gaoyang,Su, Jianguang. 2015

[9]Identification and Evaluation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis in Tea Plant (Camellia sinensis (L.) O. Kuntze). Hao, Xinyuan,Yang, Yajun,Xiao, Bin,Hao, Xinyuan,Horvath, David P.,Chao, Wun S.,Hao, Xinyuan,Yang, Yajun,Wang, Xinchao,Hao, Xinyuan,Yang, Yajun,Wang, Xinchao. 2014

[10]Screening of valid reference genes for real-time RT-PCR data normalization in Hevea brasiliensis and expression validation of a sucrose transporter gene HbSUT3. Li, Heping,Qin, Yunxia,Xiao, Xiaohu,Tang, Chaorong,Li, Heping,Qin, Yunxia,Xiao, Xiaohu,Tang, Chaorong,Li, Heping,Xiao, Xiaohu. 2011

[11]Identification of Suitable Reference Genes for Barley Gene Expression Under Abiotic Stresses and Hormonal Treatments. Hua, Wei,Zhu, Jinghuan,Shang, Yi,Wang, Junmei,Jia, Qiaojun,Yang, Jianming.

[12]Production and characterization of an amphidiploid derived from interspecific hybridization between Solanum melongena L. and Solanum aculeatissimum Jacq.. Zhou, Xiaohui,Bao, Shengyou,Liu, Jun,Yang, Yan,Zhuang, Yong. 2018

[13]Comprehensive evaluation of candidate reference genes for gene expression studies in Lysiphlebia japonica (Hymenoptera: Aphidiidae) using RT-qPCR. Xue-Ke Gao,Cui, Jin-Jie,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min Lü,Li-Juan Zhang,Xiang-Zhen Zhu,Li Wang,Hui Lu,Jin-Jie Cui.

[14]Selection of reference genes for studies of porcine endometrial gene expression on gestational day 12. Zhang, Hao. 2011

[15]Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs. Niu, Guanglin,Yang, Yalan,Zhang, YuanYuan,Hua, Chaoju,Wang, Zishuai,Tang, Zhonglin,Li, Kui,Niu, Guanglin,Yang, Yalan,Tang, Zhonglin,Li, Kui. 2016

[16]Development and application of a general plasmid reference material for GMO screening. Wu, Yuhua,Li, Jun,Wang, Yulei,Li, Xiaofei,Li, Yunjing,Zhu, Li,Li, Jun,Wu, Gang,Wu, Yuhua,Li, Jun,Wang, Yulei,Li, Xiaofei,Li, Yunjing,Zhu, Li,Li, Jun,Wu, Gang. 2016

[17]Selection of reference genes for quantitative real-time PCR in Cocos nucifera during abiotic stress. Xia, Wei,Liu, Zheng,Yang, Yaodong,Xiao, Yong,Zhao, Songlin,Mason, Annaliese S.,Mason, Annaliese S.,Ma, Zilong. 2014

[18]Selection of reliable reference genes for gene expression studies in Clonostachys rosea 67-1 under sclerotial induction. Li, Shi-Dong,Sun, Man-Hong.

[19]Analysis of multiple transcriptomes of the African oil palm (Elaeis guineensis) to identify reference genes for RT-qPCR. Xia, Wei,Xiao, Yong,Liu, Zheng,Yang, Yaodong,Lei, Xintao,Ma, Zilong,Peng, Ming,Mason, Annaliese S.,Mason, Annaliese S.,Wu, Xiaoming.

[20]Identification of suitable reference genes during the formation of chlamydospores in Clonostachys rosea 67-1. Sun, Zhanbin,Li, Shidong,Sun, Manhong. 2017

作者其他论文 更多>>

微信小程序