A Time-Course Proteomic Analysis of Rice Triggered by Plant Activator BTH

文献类型： 外文期刊

第一作者： Chen, Zhuo

作者： Chen, Zhuo;Chen, Binghua;Guo, Qin;Chen, Zhuo;Chen, Binghua;Guo, Qin;Shi, Li;He, Ming;Qin, Zhiyang;Li, Lianhua;He, Peng;Wang, Zhenchao;Hu, Deyu;Yang, Song

作者机构：

关键词： Benzothiadiazole (BTH);Label-free proteomics;Bioinformatics;Multi-reaction monitoring (MRM);Protein expression;Enzyme activity;Semi-quantitative RT-PCR

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Benzothiadiazole (BTH) is an artificial inducer of systemic acquired resistance. Due to rice being an important food crop and model plant, we investigated its response to BTH using label-free proteomics technology coupled with bioinformatics. Protein expression levels were verified using the multi-reaction monitoring mode and semi-quantitative RT-PCR. BTH treatment can up- or down-regulate many proteins produced by the rice host at all four periods, with the numbers of proteins being 6/24, 9/10, 14/10, and 8/20, respectively. Compared with mock treatments (phosphate buffered saline with 0.1 % dimethylsulfoxide and 0.5 % Tween-20), some proteins related to plant resistance were only detected after BTH treatments, such as ascorbate peroxidase (POD) 3, chitinase A, thioredoxin-dependent POD 2, beta-1,3-glucanase 2, POD superfamily protein, major facilitator superfamily (MFS) protein, copper/zinc-superoxide dismutase (SOD) 1, pathogenesis-related protein (PR) 1. Other proteins showing up-regulation after BHT treatment included PR-5, glyceraldehyde-3-phosphate dehydrogenase C, plasma-membrane associated cation-binding protein 1, and oxidoreductase family proteins. These results indicated that BTH was involved with inducing rice resistance. Some up-regulated proteins were also involved in other metabolic processes. The activity and expression level of POD, phenylalanine ammonia-lyase (PAL), and SOD, lipoxygenase (LOX), beta-1,3-glucanases, and chitinases were determined using the enzyme activity assay and semi-quantitative RT-PCR. These results indicated that BTH can enhance the activity of beta-1,3-glucanases, LOX, PAL, and POD. BTH can also induce up-regulation of the copper/zinc-SOD, ascorbate POD, glutathione POD 1, Chitinase, and LOX1 genes.

分类号： Q94

  • 相关文献

[1]Prokaryotic expression, identification and bioinformatics analysis of fbpB-esxA fusing gene from Mycobacterium tuberculosis. Wu, Qiang,Zhou, Peng,Wu, Qiang,Fu, Qiongyao,Cai, Qunfang,Fan, Zhigang,Zhan, Zhinong,Niu, Lina,Pei, Hua,Wu, Qiang,Zhou, Peng,Chen, Qian. 2011

[2]Thioredoxin reductase from Toxoplasma gondii: an essential virulence effector with antioxidant function. Xue, Junxin,Jiang, Wei,Chen, Yongjun,Wang, Minyan,Wang, Quan,Xue, Junxin,Gong, Fengju,Zeng, Peng,Xia, Can,Huang, Kehe,Xue, Junxin.

[3]NbEXPA1, an alpha-expansin, is plasmodesmata-specific and a novel host factor for potyviral infection. Li, Fangfang,Renaud, Justin,Shen, Wentao,Li, Yinzi,Guo, Lihua,Cui, Hongguang,Sumarah, Mark,Wang, Aiming,Park, Sang-Ho,Li, Yinzi,Wang, Aiming,Shen, Wentao,Guo, Lihua,Park, Sang-Ho,Cui, Hongguang. 2017

[4]Functional expression analysis of an acyl-ACP thioesterase FatB1 from Arachis hypogaea L. seeds in Escherichia coli. Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,He, Qingfang,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping. 2012

[5]Cloning and Characterization of Four New Splice Variants of Insulin-Like Growth Factor-I Gene in Chinese Red Steppes. Zhang, Jinyu,Yang, Runjun,Niu, Shuling,Liu, Dianfeng,Xing, Shenyang,Zhao, Zhihui,Zhang, Guoliang,Zhao, Yumin,Bai, Wenlin,Sun, Liang. 2011

[6]Isolation and characterization of GoRAV, a novel gene encoding a RAV-type protein in Galegae orientalis. Chen, Xiaofang,Wang, Zan,Wang, Xuemin,Gao, Hongwen,Chen, Xiaofang,Ren, Jizhou,Wang, Xuemin.

[7]Genome-wide analysis of SINA family in plants and their phylogenetic relationships. Wang, Meng,Jin, Ying,Fu, Junjie,Zhu, Yun,Hu, Jian,Wang, Guoying,Zheng, Jun,Wang, Guoying.

[8]Anatomical and physiological differences and differentially expressed genes between the green and yellow leaf tissue in a variegated chrysanthemum variety. Chang, Qingshan,Chen, Sumei,Chen, Yu,Deng, Yanming,Chen, Fadi,Zhang, Fei,Chang, Qingshan,Deng, Yanming,Wang, Shuwei.

[9]Cloning and Expression Analysis of Two MADS-Box Genes from Strawberry Fruit. Zhou, H. C.,Kang, Z. R.,Zhao, X.,Li, G.,Kang, Z. R.,Li, L..

[10]Molecular characterization and association analysis of FBXO40 with partial hematological indexes in pig. Wang, Z. W.,Li, X. Y.,Tang, Z. L.,Yang, S. L.,Ying, Z. Z.,Fu, T.,Mu, Y. L.,Ao, H.,Li, K.,Wang, Z. W.,Li, X. Y.,Ying, Z. Z.,Fu, T.,Fan, B.. 2010

[11]Expression analysis of two reverse duplicated small heat shock protein genes in rice (Oryza sativa L.). Guo Hong-xia,Zeng Wen-zhi,Feng Jing-lei,Tang Hui-wu,Bai Mei,Liu Yao-guang,Guo Jing-xin,Guo Hong-xia,Wang Chuang-yun,Zhao Li,Wang Lu-jun,Fan Tao. 2016

[12]Gene Expressing Difference in Sclerotial Formation of Morchella conica. Chen, Li-Jiao,Chen, Li-Jiao,Chai, Hong-Mei,Chen, Wei-Min,Huang, Xing-Qi,Zhao, Yong-Chang,Chai, Hong-Mei,Chen, Wei-Min,Huang, Xing-Qi,Zhao, Yong-Chang,Chai, Hong-Mei,Chen, Wei-Min,Huang, Xing-Qi,Zhao, Yong-Chang.

[13]Binding Characterization of Recombinant Odorant-binding Proteins from the Parasitic Wasp, Microplitis mediator (Hymenoptera: Braconidae). Zhang, Shuai.

[14]Molecular Cloning, and Characterization of an Adenylyl Cyclase-Associated Protein from Gossypium arboreum L.. Wang Sheng,Zhao Guo-hong,Jia Yin-hua,Du Xiong-ming. 2009

[15]Association of A-FABP gene polymorphism in intron 1 with meat quality traits in Junmu No. 1 white swine. Gao, Yan,Li, Fujuan,Wang, Shuai,Dai, Lisheng,Jiang, Hao,Liu, Dianfeng,Zhao, Zhihui,Zhang, Jiabao,Zhang, Yonghong,Sun, Boxing,Zhang, Shumin,Xiao, Shuqi. 2011

[16]Characterization of M-class genome segments of muscovy duck reovirus S14. Zhang, Yun,Guo, Dongchun,Geng, Hongwei,Liu, Ming,Hu, Qilin,Wang, Junwei,Tong, Guangzhi,Kong, Xiangang,Liu, Nihong,Liu, Chunguo. 2007

[17]Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta). Shao Zhanru,Li Qiuying,Yao Jianting,Duan Delin,Liu Fuli,Shao Zhanru,Li Qiuying. 2014

[18]Molecular cloning of a full-length cDNA for ECBP21 from Angelica dahurica. Mao, GH,Tang, WQ,Guo, Y,Ding, CB,Zhou, RG,Sun, DY. 2002

[19]Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. Chu, Xiao-Yu,Wu, Ning-Feng,Deng, Min-Jie,Tian, Jian,Yao, Bin,Fan, Yun-Liu.

[20]A novel self-cleavage system for production of soluble recombinant protein in Escherichia coli. Feng, Yufei,Wu, Donglai,Feng, Yufei,Xu, Qingyuan,Yang, Tao,Sun, Encheng,Li, Junping,Wu, Donglai,Shi, Dongfang.

作者其他论文 更多>>

微信小程序