Physiology, pathogenicity and immunogenicity of live, attenuated Salmonella enterica serovar Enteritidis mutants in chicks

文献类型: 外文期刊

第一作者: Si, Wei

作者: Si, Wei;Wang, Xiumei;Liu, Huifang;Yu, Shenye;Li, Zhaoli;Chen, Liping;Zhang, Wanjiang;Liu, Siguo

作者机构:

关键词: Chicks;Live attenuated vaccines;S. Enteritidis;Immune response

期刊名称:MICROBIAL PATHOGENESIS ( 影响因子:3.738; 五年影响因子:3.663 )

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收录情况: SCI

摘要: To construct a novel live, attenuated Salmonella vaccine, the Ion, cpxR and cpdB genes were deleted from a wild-type Salmonella enterica serovar Enteritidis-6 (SM-6) strain using the phage gimel Red homologous recombination system, resulting in SM-Delta CpxR, SM-Delta C/Lon and SM-Delta C/L/CpdB. The growth curves of strain SM-Delta C/Lon grew more rapidly than the other strains and had OD 600 values higher than the other strains starting at the 4 h time point. The growth curves of strain SM-Delta C/L/CpdB were relatively flat. The colonization time of SM-Delta C/L/CpdB is about 8-10 days. Deleting the lon/cpxR/cpdB (SM-6) genes resulted in an approximate 10(3)-fold attenuation in virulence assessed by the analysis of the LD50 of specific pathogen-free (SPF) chicks. This result indicated that the deletion of the Ion, cpxR and cpdB genes induced significant virulence attenuation. The protective effects of SM-Delta C/L/CpdB vaccination in SPF chicks against 5 x 10(9) colony forming units (CFU) of S. Enteritidis were resulted from the induction of an effective immune response. These findings demonstrate the potential of mutant SM-Delta C/L/CpdB to be used as an effective vaccine. (C) 2015 Elsevier Ltd. All rights reserved.

分类号: R3

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