A gamma-Glutamylcysteine Synthetase Gene from Pyrus calleryana Is Responsive to Ions and Osmotic Stresses

文献类型： 外文期刊

第一作者： Li, Hui

作者： Li, Hui;Han, Jin-long;Lin, Jing;Yang, Qing-song;Chang, You-hong

作者机构：

关键词： Expression regulation;Glutathione content;Pyrus calleryana;Stress tolerance;gamma-Glutamylcysteine synthetase

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

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收录情况： SCI

摘要： Pyrus calleryana Decne. is widely used as a pear rootstocks in Asia. Glutathione (GSH) can induce the phytochelatin synthase gene's (PcPCS1) transcription in this plant when exposed to cadmium. This phenomenon indicated that GSH plays a vital role in protecting P. calleryana from cadmium stress. Here, we have isolated and characterized a gene encoding.-glutamylcysteine synthetase (Pc gamma ECS), the key enzyme of GSH biosynthesis, from the leaves of P. calleryana, with the aim of expanding our knowledge of the role of GSH at the molecular level. P. calleryana plants treated with cadmium, salt, PEG6000, or mannitol responded by increasing transcription of the Pc gamma ECS gene. The response was specific for cadmium, which causes a toxicity that is thought to be mitigated through phtochelatins, and other treatments that cause damage that can be relieved by the synthesis of GSH. Feeding experiments suggested that cysteine contributed to activating the transcription of Pc gamma ECS and GSH synthesis. However, the gene's expression was inhibited in the presence of GSH. Recombinant Escherichia coli carrying Pc gamma ECS had higher GSH synthesis levels and grew better than the control cells under cadmium, salt, or osmotic stresses. These results suggest that Pc gamma ECS may participate in responses against multiple environmental stimuli in P. calleryana.

分类号： Q94`Q7

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