Expression of a scorpion toxin gene BmKit enhances the virulence of Lecanicillium lecanii against aphids

文献类型: 外文期刊

第一作者: Xie, Ming

作者: Xie, Ming;Zhang, Yan-Jun;Zhai, Xiao-Man;Zhao, Jin-Jin;Peng, De-Liang;Wu, Gang

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关键词: Biological control;Entomopathogenic fungus;Genetic engineering;Virulence

期刊名称:JOURNAL OF PEST SCIENCE ( 影响因子:5.918; 五年影响因子:5.955 )

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收录情况: SCI

摘要: Lecanicillium lecanii has been developed as biopesticides and widely used in the biological control of several insects in agricultural practice. However, the poor efficacy has blocked its application. The genetic manipulation has been proved as a useful tool to improve the virulence of entomopathogenic fungus. In this study, a scorpion toxin gene (BmKit) from Buthus martensi was cloned and transferred into L. lecanii and then its resulting activity against cotton aphids (Aphis gossypii) was assessed. The results showed that the engineered strain BmKit-12 grew significantly quicker than WT in host insects. The median lethal concentration (LC50) for BmKit-12 was 7.1-fold lower than that for WT, and the median survival time (LT50) for BmKit-12 was reduced by 26.5 % compared with that for WT. Although the conidial yield was reduced by 31.4 % on insect cadavers, the mycelia growth, sporulation, and conidia germination of BmKit-12 on plates were not significantly different from WT. Considering the lower amount of mycelia for BmKit-12 in host insects in late growth stage (fungal sporulation stage), we think the expression of BmKit may not affect the capability of sporulation. In conclusion, the expression of BmKit gene can significantly enhance the pathogenicity of L. lecanii against cotton aphids in a relatively cost-effective way.

分类号: S4

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